中国免疫学杂志Issue(9)：1186-1190,1194,6.DOI:10.3969/j.issn.1000-484X.2015.09.008
体外冲击波联合增强型纤维蛋白胶负载骨生长因子治疗兔骨不连的研究
Extracorporeal shock wave therapy plus enhanced load fibrin glue bone growth factors for nonunion or delayed osseous union of rabbit
喻绍顶 1倪卫东1
作者信息
- 1. 重庆医科大学附属第一医院骨科，重庆 400016
- 折叠
摘要
Abstract
Objective:To evaluate the effect of Extracorporeal Shock Wave combined percutaneous injection of enhanced fibrin glue loaded bone growth factor in accelerating the bone nonunion healing and promoting osteogenesis process .Methods: 48 radius model rabbits were randomly divided into four groups.Group A rabbits were treated with ESW;Group B rabbits were treated with ESW combined with FG;Group C rabbits were treated with ESW combined with FG/rhBMP-2/rhbFGF;Group D rabbits were treated with ESW combined with FG/rhBMP-2/rhbFGF/rhVEGF.After a combination therapy of 8 week,conventional X-ray radiography,optical in-spection organization form and biomechanical test were applied to evaluate the therapeutic effect .Results:Compared with the group B , the X-ray group C showed that the fracture line was fuzzy ,bony callus area began to increased ,fracture end range reduced.The histo-morphology showed that cartilage began to form , cartilage cell began to proliferate actively , and new blood vessels were forming surrounding fibrous connective tissue.Biomechanical determination found there was no statistical significance between the two groups ( P>0.05 ).FG/rhBMP-2/rhbFGF+ESW group showed the fracture line was still fuzzy , bony callus disappeared and apparent proliferative bone trabecula ,and biomechanical results showed that there was statistical significance between the two groups compared to the group B(P<0.01).FG/rhBMP-2/rhbFGF/rhVEGF+ESW group showed the fracture line was slightly fuzzy ,apparent proliferative bone trabecula in 4 week,and in 8 week,the fracture line was disappeared and moulding of healing was good.The histomorphology showed that the distribution was filled with fibrous connective tissue and many bone tissue .Biomechanical results showed that it could resistance to bending ,there was statistical significance between the two groups compared to the group C ( P<0.01 ).Conclusion: ESW therapy combined percutaneous injection of enhanced fibrin glue loaded bone growth factor can promote fracture healing and the method of combination is expected to be a good choice of non-surgical treatment of bone nonunion.关键词
体外冲击波( ESW)/骨折/骨不连/愈合/增强型纤维蛋白胶负载骨生长因子Key words
Extracorporeal shock wave/Bone fracture/Bone nonunion/Union/Enhanced fibrin glue loaded bone growth factor分类
医药卫生引用本文复制引用
喻绍顶,倪卫东..体外冲击波联合增强型纤维蛋白胶负载骨生长因子治疗兔骨不连的研究[J].中国免疫学杂志,2015,(9):1186-1190,1194,6.
