中国免疫学杂志Issue(9)：1195-1199,5.DOI:10.3969/j.issn.1000-484X.2015.09.010
红景天苷对骨髓抑制贫血小鼠骨髓细胞SP和NK-1 R表达的影响
Effects of Salidroside on expression of substance P and neurokinin-1 receptor of bone marrow cells in bone marrow depressed anemia mice
张新胜 1程航 1徐曼曼 1祝彼得2
作者信息
- 1. 河南师范大学生命科学学院，新乡 453007
- 2. 成都中医药大学，成都 610075
- 折叠
摘要
Abstract
Objective:To observe the effects of salidroside on the expression of substance P (SP)and neurokinin-1 receptor (NK-1R)of bone marrow cells(BMCs)in bone marrow(BM)depressed anemia mice,and to explore its roles for hematopoietic regulation.Methods:Automatic blood cell analysator was used to detected the changes of peripheral blood cells in each group ,immuno-histochemistry and reverse transcription-polymerase chain reaction ( RT-PCR) were used to analyze the expressions of SP and its receptor NK-1R of BMCs in each group respectively.Results: Peripheral blood testing results showed that the number of white blood cells (WBC),red blood cells(RBC)and hemoglobin(HB)of model group were significantly decreased when compared with control group.Compared with model group ,low-dose,middle-dose and high-dose salidroside obviously elevated the number of white blood cells ,and middle-dose salidroside obviously elevated the number of platelets.Immunohistochemistry studies showed that the expression of SP and its receptor NK-1R of BMCs was found in each group.Compared with control group , the expression of SP of BMCs was decreased obviously in model group(P<0.05),and the expression of NK-1R of BMCs was decreased slightly in model group (P>0.05),and the expression of SP and its receptor NK-1R of BMCs was increased significantly in low-dose,middle-dose and high-dose salidroside groups (P<0.05).RT-PCR data showed that the expression of SP mRNA of BMCs was increased obviously in model group ,low-dose,middle-dose and high-dose salidroside groups when compared with control group (P<0.05).The expression of NK-1R mRNA of BMCs was un-detected in control group and model group.The expression level of NK-1R mRNA of BMCs was elevated gradually with the increase of salidroside dosage in low-dose,middle-dose and high-dose salidroside groups.Conclusion: The mRNA and protein expressions of SP and its receptor NK-1R of BMCs were up-regulated significantly by salidroside in dose-dependent manner.These data suggest that salidroside could participate in the recovery of hematopoietic function of BM depressed anemia mice by increasing the expression of SP and its receptor NK-1R of BMCs.关键词
红景天苷/骨髓抑制/骨髓细胞/P物质/神经激肽-1受体Key words
Salidroside/Bone marrow depression/Bone marrow cell/Substance P/Neurokinin-1 receptor分类
医药卫生引用本文复制引用
张新胜,程航,徐曼曼,祝彼得..红景天苷对骨髓抑制贫血小鼠骨髓细胞SP和NK-1 R表达的影响[J].中国免疫学杂志,2015,(9):1195-1199,5.
