中国药房Issue(25)：3473-3475,3476,4.DOI:10.6039/j.issn.1001-0408.2015.25.06
苍附导痰汤对肥胖型多囊卵巢综合征模型大鼠子宫内膜及卵巢中oatp4a1表达与性激素水平的影响
The Effects of Cangfu Daotan Decoction on the Expression of oatp4a1 and Level of Sex Hormone in the Endometrium and Ovary of Rats with Obesity-type Polycystic Ovarian Syndrome
摘要
Abstract
OBJECTIVE:To explore the effects of Cangfu daotan decoction on expression of organic anion transporting poly-peptide(oatp4a1)and level of sex hormone in the endometrium and ovary tissues of rats with obesity-type polycystic ovarian syn-drome(PCOS). METHODS:Obesity-type PCOS rat models were established. 50 rats were randomized into a normal control(iso-metric normal saline)group,a model(isometric normal saline)group,a metformin(0.043 g/kg)group and Cangfu daotan decoc-tion high-dose and low-dose [5.68 and 1.42 g(medicinal materials)/kg] groups. The rats were given drugs ig once a day for 14 con-secutive days. The expression of oatp4a1 protein in the endometrium and ovaries of the rats was determined by ECL and Western blot. The levels of luteinizing hormone(LH),follicle stimulating hormone(FSH),testosterone(T)and estradiol(E2)in rat’s se-rum were detected by ELISA. RESULTS:Compared to the normal control group,those in the model group had weaker expression of oatp4a1 protein in the endometrium and ovaries,higher levels of serum LH,LH/FSH and T,lower levels of serum FSH and E2. There were statistical significances(P<0.01). Compared to the model group,those in metformin group,Cangfu daotan decoction high-dose and low-dose group had higher expression of oatp4a1 protein in the endometrium and ovaries,lower levels of LH/FSH and T,higher levels of serum E2;those in metformin group and Cangfu daotan decoction high-dose group had lower levels of serum LH,higher levels of serum FSH. There were statistical significances(P<0.01 or P<0.05). CONCLUSIONS:Cangfu daotan decoc-tion can increase the expression of oatp4a1 in the endometrium and ovaries of obesity-type PCOS model rat,alleviate phlegmy dampness and regulate the level of sex hormone.关键词
肥胖型多囊卵巢综合征/苍附导痰汤/有机阴离子转运肽/性激素/大鼠Key words
Obesity-type polycystic ovarian syndrome/Cangfu daotan decoction/Organic anion transporting polypeptide/Sex hormone/Rat分类
医药卫生引用本文复制引用
潘爱珍,陈克芳,侯祥平,李建军..苍附导痰汤对肥胖型多囊卵巢综合征模型大鼠子宫内膜及卵巢中oatp4a1表达与性激素水平的影响[J].中国药房,2015,(25):3473-3475,3476,4.基金项目
广东省中医药管理局基金（No.20141048）；广东省科技计划项目 （）
