中国药房Issue(25)：3488-3489,3490,3.DOI:10.6039/j.issn.1001-0408.2015.25.11
丹参酮ⅡA逆转多药耐药的体外效应研究
Study on in vitro Effects of TanshinoneⅡA to Reverse Multiple Drug Resistance
摘要
Abstract
OBJECTIVE:To study in vitro effects of tanshinoneⅡA to reverse multiple drug resistance. METHODS:MCF-7/ADM cells and A549/DDP cells were cultured with 0 [20 mg/L doxorubicin(ADM)or cisplatin(DDP),negative control],5 mg/ml tanshinone ⅡA(combined with 20 mg/L ADM or DDP)for 24 and 48 h. Then MTT method was used to determine cell viability, and polymerase chain reaction(PCR)was adopted to detect mRNA expressions of cell cycle control protein CDC25A and cell cy-clin dependent kinase (CKD2). RESULTS:Compared to the negative control,after MCF-7/ADM cells and A549/DDP cells were cultured with tanshinone ⅡA for 24 and 48 h,cell viability was weaker,also were mRNA expressions of CDC25A and CKD2. There were statistical differences(P<0.01). CONCLUSIONS:Tanshinone ⅡA combined with ADM or DDP can inhibit the viabili-ty of cell line MCF-7/ADM and cell line A549/DDP,decrease expression of CDC25A,CKD2 mRNA in cells and reverse multiple drug resistance in malignant tumors.关键词
丹参酮ⅡA/多药耐药/肺癌/乳腺癌/细胞活力Key words
TanshinoneⅡA/Multiple drug resistance/Lung cancer/Breast cancer/Cell viability分类
医药卫生引用本文复制引用
潘登,陈可和..丹参酮ⅡA逆转多药耐药的体外效应研究[J].中国药房,2015,(25):3488-3489,3490,3.基金项目
广西壮族自治区卫生厅自筹经费科研课题 （）
