摘要
Abstract
OBJECTIVE:To study the induction of ethyl acetate extract from Actinidia arguta on apoptosis of lung cancer A549 cells. METHODS:After the cells were cultured in 0(negative control)40,80 and 160μg/ml ethyl acetate extract from A. ar-guta for 48 and 72 h,gel electrophoresis method was used to detect DNA cleavage in the cells. After the cells were cultured in 0 (negative control),40,80 and 160 μg/ml ethyl acetate extract from A. arguta for 24,48 and 72 h,flow cytometry was adopted to detect apoptosis and cell cycle distribution,and immunohistochemical method was employed to detect Survivin expression. RE-SULTS:After 48 and 72 h culture in 40,80 and 160 μg/ml ethyl acetate extract from A. arguta,ladders appeared,which are the characteristic of apoptosis. Compared to the negative control,following 24,48 and 72 h culture of cells in 40,80 and 160 μg/ml ethyl acetate extract from A. arguta,apoptosis rate was higher,also was the percentage of the cells in G0/G1 phase;and the expres-sion of Survivin was weaker,demonstrating a time and concentration-dependent relation. CONCLUSIONS:The ethyl acetate ex-tract from A. arguta can induce apoptosis of A549 cells and cause a cell cycle arrest in G0/G1 phase,by a mechanism which may be related to the reduction in Survivin expression.关键词
肺癌A549细胞/藤梨根乙酸乙酯提取物/生存素/诱导凋亡Key words
Lung cancer A549 cells/Ethyl acetate extract from Actinidia arguta/Survivin/Apoptosis induction分类
医药卫生
