中国药理学通报Issue(9)：1249-1253,5.DOI:10.3969/j.issn.1001-1978.2015.09.014
氯通道在阿霉素诱导鼻咽癌细胞凋亡中的作用
Roles of chloride channels in apoptosis induced by adriamycin in nasopharyngeal carcinoma cells
摘要
Abstract
Aim To investigate the roles of chloride channels in the apoptosis and apoptotic volume de-crease (AVD)induced by adriamycin in nasopharyn-geal carcinoma CNE-2Z cells.Methods Apoptotic rates were detected by flow cytometry,and the volume changes were measured by the time-lapse live cell ima-ging technique.The patch clamp technique was used to record whole-cell chloride currents.Results Adria-mycin induced apoptosis of CNE-2Z cells.An early ap-optotic volume decrease was observed in the cell trea-ted with adriamycin.The cell volume was decreased by about 10% in 2 h.Adriamycin activated a chloride current which showed outward rectification.The chlo-ride channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB ) could inhibit the adriamycin-in-duced chloride currents,apoptosis and prevent cell shrinkage.Conclusions Our findings suggest that ad-riamycin causes cell apoptosis by activation of chloride channels.Chloride channels may be involved in the apoptosis and apoptotic volume decrease induced by adriamycin in CNE-2Z cells.关键词
阿霉素/鼻咽肿瘤/氯通道/膜片钳技术/凋亡/凋亡性容积减小Key words
adriamycin/nasopharyngeal neoplasms/chloride channels/patch clamp technique/apoptosis/apoptotic volume decrease分类
医药卫生引用本文复制引用
刘梅,王立伟,陈丽新,罗海,林嘉伟,魏燕,李媛,刘善文,孟龙,邹丽莉,朱林燕..氯通道在阿霉素诱导鼻咽癌细胞凋亡中的作用[J].中国药理学通报,2015,(9):1249-1253,5.基金项目
国家自然科学基金资助项目（No 81273539，81173064，81272223）；教育部博士点基金资助项目（No 20124401110009）；广州市科技计划项目（No 2013J500015）；东莞市科技计划项目（No 2011108102006）；湖南省教育厅资助科研项目 （）
