中国癌症防治杂志Issue(4)：244-249,250,7.DOI:10.3969/j.issn.1674-5671.2015.04.02
慢病毒介导过表达组织蛋白酶S基因对肝癌MHCC97H细胞增殖和迁移的作用
Effects of lentivirus-mediated overexpression of Cat S gene on proliferation and migration of hepatocellular carcinoma cell MHCC97H
摘要
Abstract
Objective To construct a lentivirus expression vector to drive stable Cat S overexpression in the hepatocellular carcinoma (HCC)cell line MHCC97H,and to investigate the effects of overexpression on cell proliferation and migration. Methods The Cat S gene was inserted into the lentiviral expression vector pLVX-EGFP-3FLAG-Puro using Age I and EcoR I restriction enzymes and transfected into 293T cells to generate recombinant lentivirus. This virus was used to infect MHCC97H cells,and positive cells were selected using puromycin. Real-time quantitative PCR,Western blots,MTT assay,the transwell migration assay and the wound healing migration assay were used to assess the effects of Cat S overexpression on proliferation and migration of MHCC97H cells. Results A lentiviral vector containing the Cat S gene was constructed,and an MHCC97H cell line stably overexpressing Cat S was established. Cat S overexpression was associated with significantly shorter cell doubling time,as well as significantly greater invasion and migration abilities. Conclusions Lentivirus-mediated Cat S overexpression can increase MHCC97H proliferationand migration,opening the door to future studies of molecular mechanisms of liver cancer invasion and metastasis.关键词
肝肿瘤/组织蛋白酶S/过表达/慢病毒/增殖/迁移Key words
Liver neoplasm/Cathepsin S/Overexpression/Lentivirus infections/Proliferation/Migration分类
医药卫生引用本文复制引用
张智,朱广志,彭涛,赵国良,徐静..慢病毒介导过表达组织蛋白酶S基因对肝癌MHCC97H细胞增殖和迁移的作用[J].中国癌症防治杂志,2015,(4):244-249,250,7.基金项目
国家自然科学基金资助项目（81360372）；广西自然科学基金资助项目（2011GXNSFA018284）；广西卫生厅科研基金资助项目 （）
