广东海洋大学学报Issue(4)：1-9,9.
卵形鲳鲹 PPAR α基因 cDNA 序列的克隆、组织表达及生物信息学分析
Molecular Cloning and Expression Distribution and Bioinformatics Analysis of Peroxisome Proliferator Activated Receptors-α in Trachinotus ovatus
摘要
Abstract
The full-length cDNA of PPAR α in Trachinotus ovatus was cloned by RACE (Rapid Amplification of cDNA Ends), and the protein structures, physical, chemical properties and their functional domain structures which encoded by PPAR α gene were analyzed by bioinformatics method. The results showed that cDNA sequences of PPAR α in T. ovatus were 1 930 bp, and the open reading frame was 1 425 bp, encoding 474 amino acids (GenBank accession No. KP893147). The protein was unstable protein without signal peptide and trans-membrane. The secondary structure of PPAR αcontains alpha helixes, beta turns, and extended strands, random coils. At the same time, alpha helixes were 45.15%. There were some typical structures in PPAR α, including a DNA-binding domain with zinc-finger structures, and a ligand binding domain which can integrate ligands and activate PPAR α. That the sequences of T. ovatus PPAR α were highly homologous with those cloned from Japanese sea perch, cobia, large yellow croaker and gilthead sea-bream (81% - 89%), phylogenetic tree analysis of PPAR α protein showed that the nearest relationship existed between T. ovatus and cobia among all the species mentioned above, and the lowest homology was human. Using quantitative real-time RT-PCR, we observed that PPAR α mRNA was expressed predominantly in brain-lipid, spleen, intestine and head-kidney tissues of T. ovatus.关键词
卵形鲳鲹/过氧化物酶体增殖物激活受体 α(PPAR α)/基因/克隆/cDNA 末端快速扩增(RACE)/生物信息学/组织表达Key words
Trachinotus ovatus/peroxisome proliferator activated receptors-α (PPAR α)/gene/clone/Rapid Amplification of cDNA Ends (RACE)/bioinformatics/tissue expression分类
生物科学引用本文复制引用
方玲玲,陈刚,王忠良,汤保贵,张健东,黄建盛,周晖..卵形鲳鲹 PPAR α基因 cDNA 序列的克隆、组织表达及生物信息学分析[J].广东海洋大学学报,2015,(4):1-9,9.基金项目
国家海洋公益性行业科研专项（201205028）；广东省海洋经济创新发展区域示范专项（GD2012-A01-007，GD2012-A02-003）；广东省教育厅创新计划专项（2012KJCX0063）；广西科技厅科技计划 （）
