广东海洋大学学报Issue(4)：17-23,7.
间氨基苯甲酸乙酯甲磺酸盐对泥鳅的麻醉效果
Anesthetic Effects of 3-Aminobenzoic Acid Ethyl Ester Methanesulfonate (MS-222) on Misgurnus anguillicaudatus
摘要
Abstract
Based on the stress reaction of the loach (Misgurnus anguillicaudatus) behavior characteristics and respiratory frequency at 20-140 mg/L concentrations by 3-Aminobenzoic acid ethyl ester methanesulfonate (MS-222), the final anesthetic status can be divided into six stages, and the revival process into four stages. The anesthetic effects of MS-222 on loach was studied under the conditions of different MS-222 concentrations (140 - 340 mg/L) and water temperature (12 and 22 ℃), and different weights (5.1, 11.6 and 28.1 g) of the loach at 180 mg/L concentration of MS-222. The results showed that the loach can reach anesthesia stage 3 at 80 mg/L of MS-222 solution concentration, and it can be a reference concentration for loach long-distance transportation; The breath frequency of loach showed no significant changes when the loach were in the final anesthetic stages 1 to 3, and <br> declined clearly until stage 4 under the concentration range of 20 - 140 mg/L. The induction time of the loach under the condition of temperature 12 ℃,in the MS-222 solution with mass concentration of 140- 340 mg/L, were longer than those under the condition of temperature 22 ℃, and the change of recovery time was same to induction time. The effective anesthetic concentrations of MS-222 on loach at 12 and 22 ℃ are 180, 220 mg/L, respectively. Small loach individuals have short anesthesia time and long recovery time (P < 0.05). It is suggested that MS-222 has preferable anesthetic effect to the loach, and at the anesthesia operation, we need to consider factors such as water temperature, fish size in order to use reasonable anesthesia concentration for the loach.关键词
泥鳅/麻醉/间氨基苯甲酸乙酯甲磺酸盐(MS-222)Key words
Misgurnus anguillicaudatus/anesthesia/3-Aminobenzoic acid ethyl ester methanesulfonate (MS-222)分类
农业科技引用本文复制引用
韩光明,毕建花,张家宏,寇祥明,朱凌宇,徐荣,王桂良,王守红..间氨基苯甲酸乙酯甲磺酸盐对泥鳅的麻醉效果[J].广东海洋大学学报,2015,(4):17-23,7.基金项目
江苏省农业科技支撑计划项目（BE2014360）；江苏里下河地区农科所基金项目 （）
