肿瘤药学Issue(4)：270-274,5.DOI:10.3969/j.issn.2095-1264.2015.04.07
吡格列酮对人胃癌 SGC7901细胞增殖和凋亡的影响
Effects of Pioglitazone on the Proliferation and Apoptosis of Human Gastric Cancer Cell Line SGC7901
摘要
Abstract
Objectives To research the effects of pioglitazone on SGC7901 human gastric cancer cell line. Methods After induced by 5 ng·mL-1 TGF-β1, the SGC7901 human gastric cancer cell line in the research groups were treated by pioglitazone with different concentrations (5, 10, 20, 25 μmol·L-1) for different times (0, 12, 24, 48, 72 h). The control group had no medicament of pioglitazone. Then the proliferation and apoptosis of gastric cancer cells was detected respectively by MTT and flow cytometry method. The real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was applied to detect the gene expression level of peroxisome proliferator-activated receptorγ (PPARγ). Results Within the concentration range of 5~40 μmol·L-1, pioglitazone inhibited the growth of 5 ng·ml-1 TGF-β1 induced SGC7901 cells in a dose and time dependent manner (P<0.05). After treated by 10 μmol·L-1 of pioglitazone for 12 h, the apoptosis rate of TGF-β1 induced SGC7901 cell was increased significantly as compared with the con-trol group, and it was increased gradually along with the increase of the dose and time and reached the highest at 72 h (P<0.01). The PPARγmRNA expression level was also gradually upregulated along with the increase of the concentration of pioglitazone and treat-ing time, and difference had statistical significance (P<0.05). Conclusion Pioglitazone could inhibit the growth and promote the ap-optosis of TGF-β1 induced gastric cancer SGC7901 cells in a dose and time dependent manner through upregulating the expression of the PPARγ.关键词
吡格列酮/胃癌/SGC7901细胞株/增殖/凋亡Key words
Pioglitazone/Gastric Cancer/SGC7901 cell line/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
陈静波,陈巧,郑建萍,崔同建,林俊锦,刘振华,李德育,张桂枫,蒋桂成,陈峥..吡格列酮对人胃癌 SGC7901细胞增殖和凋亡的影响[J].肿瘤药学,2015,(4):270-274,5.基金项目
福建省卫生厅青年科研课题（2009-2-6）。 （）
