作物学报Issue(10)：1472-1480,9.DOI:10.3724/SP.J.1006.2015.01472
兼抗型成株抗性小麦品系的培育、鉴定与分子检测
Development, Field and Molecular Characterization of Advanced Lines with Pleiotropic Adult-Plant Resistance in Common Wheat
摘要
Abstract
Stripe rust, leaf rust, and powdery mildew are devastative fungal diseases of common wheat (Triticumaestivum L.) in China, and breeding cultivars with pleiotropic adult-plant resistance is believed to be the most important solution to control these diseases effectively and environmental friendly. A total of 21 winter wheat advanced lines and 96 spring wheat advanced lines collected from adult-plant resistance breeding programs were used to estimate the level of resistance against the stripe rust, leaf rust and powdery mildew across several environments. Simultaneously, the distribution of pleiotropic resistance genes Lr34/Yr18/Pm38,Lr46/Yr29/Pm39, andSr2/Yr30 were also detected using molecular marker closely linked to the target genes. The field test showed that 17 winter wheat lines (80.9%) and 85 spring wheat lines (88.5%) performed acceptable resistance against the three diseases. All the 21 winter wheat lines tested containQPm.caas-4DL, of which seven contain QPm.caas-2BS and nine containQPm.caas-2BL. Among the 96 spring wheat lines, 18 carryLr34/Yr18/Pm38, 37 carryLr46/Yr29/Pm39, and 29 lines possessSr2/Yr30. These results indicate that molecular-marker-assistant selection in combination with conventional breeding is effective and applicable in developing pleiotropic adult-plant resistance cultivars, which provides a new thought for wheat resis-tance breeding.关键词
普通小麦/兼抗型/成株抗性/条锈病/叶锈病/白粉病Key words
Triticum aestivum L./Pleiotropic resistance/Adult-plant resistance/Stripe rust/Leaf rust/Powdery mildew引用本文复制引用
刘金栋,何中虎,杨恩年,肖永贵,陈新民,伍玲,白斌,李在峰,Garry M. Rosewarne,夏先春..兼抗型成株抗性小麦品系的培育、鉴定与分子检测[J].作物学报,2015,(10):1472-1480,9.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2013CB127700),国家自然科学基金项目(31261140370),引进国际先进农业科学技术计划(948计划)项目和科技部国际科技合作项目(2012DFA32290)资助。 （973计划）
