作物学报Issue(10)：1603-1611,9.DOI:10.3724/SP.J.1006.2015.01603
水稻黄绿叶突变体ygl209的遗传分析与目标基因精细定位
Genetic Analysis and Fine Mapping of Yellow-Green Leaf Mutantygl209 in Rice
摘要
Abstract
Etiolation mutants of rice play an important role in studies on the photosynthesis, chloroplast development, and chlo-rophyll metabolism in higher plants. Ajaponica rice mutantygl209 with yellow-green leaf was identified from the BC4F3 progeny of the cross between the transgenic variety of Zhongguo 91 and Zhendao 88 with the latter as the recurrent parent. Compared with the wild-type parent Zhendao 88, the contents of chlorophylla, chlorophyllb and carotenoid decreaseddramatically in the mutant ygl209at the seedling, tillering and heading stages, respectively. In particular, chlorophyllb decreased most significantly. How-ever, there was no significant change in other agronomic traits, such as heading stage, plant height, number of effective panicles per plant, number of grains in main stem panicle, seed setting rate and 1000-grain weight. Genetic analysis showed that the yel-low-green leaf trait of the ygl209 mutant was controlled by one pair of recessive nuclear genes. With F2 and F3segregation popu-lations derived from the cross betweenygl209 and Zhendao 88, theYGL209 gene was mapped to the centromere region of chro-mosome 1, with a physical distance of 571.6 kb. We further analyzed the putative candidate genes in the target region through sequencing. A single base substitution (G1390C) was detected in the coding region of theLOC_Os01g31110 gene, which resulted in a missense mutation (A348G) in its encoded protein. Bioinformatic analysis predicted that theLOC_Os01g31110gene is related to the chloroplast development in rice. Therefore,LOC_Os01g31110 is likely to be the candidate gene ofYGL209.关键词
水稻/黄绿叶突变体/遗传分析/精细定位Key words
Rice (Oryza sativa L.)/Yellow-green leaf mutant/Genetic analysis/Fine mapping引用本文复制引用
李广贤,姚方印,侯恒军,孙召文,姜明松,朱文银,周学标..水稻黄绿叶突变体ygl209的遗传分析与目标基因精细定位[J].作物学报,2015,(10):1603-1611,9.基金项目
本研究由山东省现代农业产业技术体系水稻产业创新团队建设项目(SDAIT-01-016-01),山东省农业良种工程项目(2013-2015)和山东省农业科学院科技创新重点项目(2014CXZ11)资助。 （SDAIT-01-016-01）
