中外医学研究2015，Vol.13Issue(23)：1-3,3.DOI:10.14033/j.cnki.cfmr.2015.23.001
DYRK1A表达与难治性颞叶癫痫的相关性研究
The Correlation Research of DYRK1A Expression and Refractory Temporal Lobe Epilepsy
摘要
Abstract
Objective:To establish a mice model of human temporal lobe epilepsy, lithium chloride-pilocarpine can be induced status epilepticus seizures in Kunming mice by intraperitoneal injection.The analysis of DYRK1A mRNA expression in mice's brain tissue is used to investigate whether DYRK1A participate in the formation of hippocampal sclerosis of intractable temporal lobe epilepsy.Method: Epileptic group were established status epilepticus (SE)by injecting a small dose of lithium chloride-pilocarpine repeatedly and intraperitonealy.We randomly selected 6 mice into acute epileptic group,6 mice into chronic epileptic group. On the basis of behavioral observation,electrophysiological , pathological HE staining method,we verified the reliability of the model and established mice model of human temporal lobe epilepsy.Reverse transcription PCR method was used to detect DYRK1A mRNA expression levels in the brain tissue of model mice.Result:There were 34 Kunming mice,which were be induced into status epilepticus seizures by intraperitoneal injection of lithium chloride-pilocarpine.22 Kunming mice were established status epilepticus (SE) and reached Racine Standard Ⅳ-Ⅴ level,6 mice were randomly selected into acute epilepsy group;The remaining 8 mice were observed within 30 days and 6 mice were selected into chronic epilepsy group;6 mice in the control group were intraperitonealy injected by saline.They were not induced into any seizure and didn't die in the next 30 days.In this study,a success rate of induced SE was 64.71%(22/34),the mortality rate after SE was 36.36%(8/22);spontaneous seizures occurred at the rate of 47.06%(8/17).Pathology HE staining:in comparison with the control group,hippocampal neurons of epileptic group arranged irregularly and the gap between neurons increased,with cell swelling,degeneration,necrosis,disintegration,cell body pyknosis, smaller volume, cytoplasm condensed hyperchromatic,nucleus pycnosis,unclear nucleoli,splitting decomposition of dead cell nuclei and cytoplasm.EEG monitoring of epileptic mice models:in the acute phase,the outbreak of the long-range sharp activities,sharp spike activity and slow irregular composite activity was prominent in the background EEG activity;in the chronic phase, scattered frequency activities slower than the background slow activity and epileptic discharge appeared.In 6 control group,DYRK1A mRNA and β-actin ratio was 0.4830±0.1243;in 6 acute epileptic group,DYRK1A mRNA and β-actin ratio was 0.8883±0.0727;in 6 chronic epileptic group,DYRK1A mRNA and β-actin ratio was 0.7112±0.1216;The ratio differences between three groups all were statistically significant(P<0.05).DYRK1A mRNA expression in three groups:acute cause epilepsy group>chronic epilepsy group>control group. Conclusion:Lithium chloride-pilocarpine can be used to induce status epilepticus(SE) in Kunming mice.In comparison with control group,DYRK1A expression in brain tissue of SE Kumming mice model increased.关键词
DYRK1A/难治性颞叶癫痫/海马硬化Key words
DYRK1A/Intractable temporal lobe epilepsy/Hippocampal sclerosis分类
医药卫生引用本文复制引用
刘肇绩,王文杰,傅俊华,彭锋,唐荣,张岱威,郑维红..DYRK1A表达与难治性颞叶癫痫的相关性研究[J].中外医学研究,2015,13(23):1-3,3.基金项目
2011年福建省卫生厅青年课题(项目编号:2011-2-67) 2012厦门市科技指导性项目(项目编号:2011S0351) （项目编号:2011-2-67）
