按摩与康复医学Issue(17)：135-138,4.
电针对大鼠退变腰椎间盘组织IL-1β、MMP-1及TIMP-1表达的影响
Effects of Electroacupuncture on the Expression of IL-1β、MMP-1、TIMP-1 and Histomorphology of Degenerative Lumbar Intervertebral Disc in Rats
摘要
Abstract
Objective:Through the establishment of rat model of lumbar disc degeneration, observed in rat lumbar disc tissue morphological changes and the impact on mRNA expression of interleukin-1β(IL-1β), matrix metalloproteinase enzyme-1 (MMP-1) and tissue inhibitor of matrix metallo-proteinase enzyme-1 (TIMP-1) by electroacupuncture stimulation. To explore the electroacupuncture treatment of lumbar degenerative disease onset mechanism, for the clinical treatment of lumbar degenerative disease provide a theoretical basis by electroacupuncture. Methods: The 40 SD rats were randomly divided into four groups as normal group, model group, electroacupuncture stimulate“Weizhong”group, electroacupuncture stimu-late non-acupoint group. The normal group were routine to fed, and the remaining three groups establishment of rat model of lumbar disc degenera-tion by puncture the annulus. Four weeks after modeling, the model group did not interfere with electroacupuncture, the“Weizhong”group and the non-acupoint group electroacupuncture stimulated the corresponding parts. Each time treated 20min, once a day, ten times as a course of treatment, lasted three courses. After finishing treatment, the rats were sacrificed, lumbar intervertebral disc tissue were removed. Rat lumbar disc tissue mor-phological changes were observed by HE staining, and the expression levels of IL-1β, MMP-1, and TIMP-1 were applied by RT-PCR technology. Re-sult:On histological, the degree of lumbar disc degeneration from light to heavy followed normal group, electroacupuncture stimulate“Weizhong”group, electroacupuncture stimulate non-acupoint group, model group. Compared model group, electroacupuncture stimulate“Weizhong”group and electroacupuncture stimulate non-acupoint group with the normal group, the mRNA expression of IL-1Β,MMP-1 were increased, the mRNA expres-sion of TIMP-1 were decreased. Compared electroacupuncture stimulate“Weizhong”group and electroacupuncture stimulate non-acupoint group with the model group, the mRNA expression of IL-1Β, MMP-1 were decreased, the mRNA expression of TIMP-1 were increased. Compared elec-troacupuncture stimulate“WeiZhong”group with the electroacupuncture stimulate non-acupoint group, the mRNA expression of IL-1Β, MMP-1 were decreased, the mRNA expression of TIMP-1 were increased. Compare with a significant difference between the groups (P<0.05). Conclusion:Electroacupuncture can reduce IL-1β, MMP-1, up-regulated TIMP-1, to delay the degeneration of intervertebral disc tissue. And electroacupuncture stimulation Weizhong acupoint stimulation than non-acupoint is more significant.关键词
腰椎间盘退变/电针/委中/IL-1β/MMP-1/TIMP-1/基因表达/大鼠Key words
lumbar disc degeneration/electroacupuncture/Weizhong/IL-1β/MMP-1/TIMP-1/gene expression/rats分类
医药卫生引用本文复制引用
林建平,连晓阳,林先钊,陈少清..电针对大鼠退变腰椎间盘组织IL-1β、MMP-1及TIMP-1表达的影响[J].按摩与康复医学,2015,(17):135-138,4.基金项目
福建省自然科学基金，编号2014J01346；福建省教育厅A类科技项目，编号JA13163；福建省卫生厅青年科研课题，编号2013-2-70 （）
