北京中医药大学学报Issue(7)：457-461,5.DOI:10.3969/j.issn.1006-2157.2015.07.007
隐丹参酮对人胃癌细胞 SGC-7901凋亡的影响及其机制
Effect and mechanism of cryptotanshinone on apoptosis of human gastric cancer cell line SGC-7901
摘要
Abstract
Objective To investigate the apoptosis effect of cryptotanshinone (CPT)on human gastric cancer cell line SGC-7901,so as to explore the molecular mechanism related.Methods SGC-7901 cells were treated with varying concentration of CPT.MTT assays and flow cytometry were used to evalu-ate the effects of CPT on the proliferation and apoptosis of SGC-7901 cells.The protein expressions of Bax,Bcl-2,p53 and p21,a series of apoptosis-related proteins,were observed by Western blotting,and the mRNA expressions of Bax,Bcl-2,p53 and p21were determined by real-time RT-PCR.Results Fol-lowing 24 h treatment,CPT was capable of inhibiting proliferation and inducing apoptosis of SGC-7901 cells significantly.In addition,Bax and p53 mRNA and protein were upregulated,while Bcl-2 mRNA and protein were downregulated.It is well known that both Bcl2 and Bax are transcriptional targets for the tumour suppressor protein,p53,which induces cell cycle arrest or apoptosis.Conclusion Apoptosis can be induced in SGC-7901 cells by CPT,potentially via a mitochondrial pathway.关键词
隐丹参酮/人胃癌细胞 SGC-7901/细胞增殖/细胞凋亡/线粒体途径Key words
cryptotanshinone/human gastric cancer cell line SGC-7901/cell proliferation/apoptosis/mitochondrial pathway分类
医药卫生引用本文复制引用
邓凤春,于占江,杨钰,赵红晔,王滨,周丽..隐丹参酮对人胃癌细胞 SGC-7901凋亡的影响及其机制[J].北京中医药大学学报,2015,(7):457-461,5.基金项目
黑龙江省教育厅科学技术研究项目 （）
