北京中医药大学学报Issue(8)：515-518,528,5.DOI:10.3969/j.issn.1006-2157.2015.08.002
肝郁、脾虚和肝郁脾虚证模型大鼠行为学变化及柴疏四君子汤对其作用的比较
Behavioral changes in rats of liver-stagnation pattern,spleen-deficiency pattern and liver-stagnation with spleen-deficiency pattern and effects of Chaihu Sijunzi Decoction on different patterns
摘要
Abstract
Objective To compare the behavioral changes of different rat models of liver-stagnation pattern,spleen-deficiency pattern,and liver-stagnation and spleen-deficiency pattern and to explore the effects of Chaihu Sijunzi Decoction (Bupleurum with Four Gentlemen Decoction)on those rat models. Methods Male Wistar rats were randomly assigned into the normal group (group 1 ),liver-stagnation group (group 2 ),spleen-deficiency group (group 3 ),liver-stagnation with spleen-deficiency group (group 4),liver-stagnation intervention group (group 5),spleen-deficiency intervention group (group 6)and liver-stagnation with spleen-deficiency intervention group (group 7)with thirteen rats in each group.The models were established by using chronic restraint method in group 2 and 5,by using excessive fatigue plus irregular diet method in group 3 and 6,and by using chronic restraint-excessive fatigue-irregular diet method in group 4 and 7 for 4 weeks.At the end of the 15 th day,rats in the group 5,6 and 7 were fed with Chaihu Sijunzi Decoction per 2.86 g·kg -1 for two weeks,whereas the rats in the normal group and model groups 2,3 and 4 were fed with equivalent saline for two weeks.On the 14th and 28 th day,open-fieldtest was used to observe the crossed squares (index C),number of rearing (index N),time remaining in the central zone (index S),decorating time (index D)and the total distance of movement (index T)of all rats.Results On the 14th day,as compared with group 1,index C,index N and index T increased significantly in group 2 and 3 (P <0.05)and decreased significantly in group 4 (P <0.05).Index S increased significantly in group 2 and 4 (P <0.05)while it decreased significantly in group 3 (P <0.05).Index D decreased significantly in group 2 and 4(P <0.05)and increased significantly in group 3 (P <0.05).On the 28 th day,compared with group 1,index C,index T decreased significantly in group 2,3 and 4 (P <0.05 ),while index N and index D decreased significantly and index S increased significantly in group 2 and 4 (P <0.05),which were totally opposite to group 3.Compared with group 2,index C and index T increased significantly while index S decreased significantly in group 5 (P <0.05).Compared with group 3,index C,index D,and index T increased significantly while index N decreased significantly in group 6(P <0.05).Compared with group 4,the crossed squares,number of rearing,decorating time,and index T increased significantly in group 7 (P <0.05).Conclusion The autonomic activities were at different degrees in rat models of liver-stagnation pattern,spleen-deficiency pattern,and liver-stagnation with spleen-deficiency pattern.There were decreased CNS excitement in the liver-stagnation pattern,disordered central nervous response in the spleen-deficiency pattern,and inhibited central excitatory activities in the liver-stagnation and spleen-deficiency pattern.Chaihu Sijunzi Decoction can improve the autonomic activities of those rat models,es-pecially of the liver-stagnation with spleen-deficiency model.关键词
肝郁/脾虚/肝郁脾虚/柴疏四君子汤/旷场实验/大鼠Key words
liver-stagnation/spleen-deficiency/liver-stagnation and spleen-deficiency/Chaihu Sijunzi Decoction/open-fieldtest/rats分类
医药卫生引用本文复制引用
赵荣华,谢鸣,刘进娜,李聪,张敬升,王帮众,么元超..肝郁、脾虚和肝郁脾虚证模型大鼠行为学变化及柴疏四君子汤对其作用的比较[J].北京中医药大学学报,2015,(8):515-518,528,5.基金项目
国家自然科学基金面上项目 （）
