Journal of Changshu Institute of Technology2015，Vol.29Issue(4)：1-7,7.
产丙酮酸氧化酶重组大肠杆菌的发酵过程质粒稳定性研究
A Study of Plasmid Stability of Recombinant E.coliDH5α/pSMLPyOD Dduring the Process of Fermentation
赵劼 1王永红 1储炬 1张嗣良 1庄英萍1
作者信息
- 1. 华东理工大学 生物工程学院,上海 200237
- 折叠
摘要
Abstract
The influences of carbon resource, temperature, DO, pH and heterologous pyruvate oxidase expression on the plasmid stability of the recombinant E.coli DH5α/pSMLPyOD were investigated. The results show that glycerol, 37℃ and the ratio of 10-15% medium to volume of the flask (v) were the optimum conditions for the plasmid stability during the process of recombinant E.coli DH5α/pSMLPyOD cultivation, that the recombinant could retain high plasmid stability in pH6-7 and that heterologous PyOD expression decreased the plasmid sta?bility apparently.关键词
丙酮酸氧化酶/重组大肠杆菌/DH5α/pSMLPyOD/质粒稳定性Key words
pyruvate oxidase/recombinant Escherichia coli/DH5α/pSMLPyOD/plasmid stability分类
生物科学引用本文复制引用
赵劼,王永红,储炬,张嗣良,庄英萍..产丙酮酸氧化酶重组大肠杆菌的发酵过程质粒稳定性研究[J].Journal of Changshu Institute of Technology,2015,29(4):1-7,7.
