南方医科大学学报2015，Vol.35Issue(8)：1110-1115,6.
原花青素上调let-7a抑制胰腺癌AsPC-1细胞生长及迁移
Proanthocyanidins inhibit pancreatic cancer AsPC-1 cell growth and migration through up-regulation of let-7a
摘要
Abstract
Objective To ascertain whether proanthocyanidins inhibit cell growth and migration by increasing let-7a expression in pancreatic cancer AsPC-1 cells. Methods The proliferation rate, cell apoptosis rate and cell migration ability of AsPC-1 cells treated with proanthocyanidins were measured by MTT assay, Annexin V-FITC/PI staining, and Transwell migration assay, respectively. The expression of let-7a AsPC cells was detected by miRNA real-time RT-PCR after proanthocyanidins treatment. The changes in the biological behaviors of AsPC-1 cells were evaluated after transfection with let-7a mimics. Results Compared with the control group, proanthocyanidins treatment caused dose-dependent decrements of the proliferation rate and migration ability and increased the apoptosis rate in AsPC-1 cells. AsPC-1 cells with proanthocyanidins treatment showed increased expression of let-7a. Transfection with let-7a mimics resulted in obvious decreases in the cell growth rate and migration ability, and proanthocyanidins treatment significantly enhanced the inhibitory effect of let-7a mimics. Conclusions Proanthocyanidins-induced cell growth and migration inhibition are partially mediated by up-regulation of let-7a expression in AsPC-1 cells.关键词
原花青素/let-7a/胰腺癌细胞Key words
proanthocyanidins/let-7a/pancreatic cancer cells引用本文复制引用
马佳,方斌斌,马聪,庞海杰,曾凡鹏,夏俊..原花青素上调let-7a抑制胰腺癌AsPC-1细胞生长及迁移[J].南方医科大学学报,2015,35(8):1110-1115,6.基金项目
国家自然科学基金(81172087) （81172087）
安徽省教育厅自然科学研究重点项目(KJ2014A153) Supported by National Natural Science Foundation of China (81172087). （KJ2014A153）
