南方医科大学学报2015，Vol.35Issue(8)：1137-1142,6.
兔抗弓形虫Ⅰ型液泡型质子焦磷酸酶多克隆抗体的制备及鉴定
Preparation and identification of rabbit polyclonal antibodies against vacuolar proton pyrophosphatase type I of Toxoplasma gondii
摘要
Abstract
Objective To prepare and characterize rabbit polyclonal antibodies against Toxoplasma gondii vacuolar proton pyrophosphatase type I (TgVP1). Methods and Results Two synthesized peptides TgVP1-1 and TgVP1-2 as the haptens were conjugated with KLH to immunize rabbits. Indirect ELISA showed that the titers of rabbit anti-TgVP1-1 polyclonal antibody and rabbit anti-TgVP1-2 polyclonal antibody reached 1:128 000. Western blotting results revealed that both purified polyclonal antibodies could specifically bind to a purified 85 kD T. gondii protein predicted as TgVP1. The protein detected by these two polyclonal antibodies was distributed in the cytoplasm of T. gondii tachyzoite, and this distribution pattern was consistent with that of acidocalcisome. Conclusion The peptide-based method of antibody generation is efficient and the obtained TgVP1 polyclonal antibodies possess a high specificity to facilitate further study of T. gondii acidocalcisome and the diagnosis of toxoplasmosis.关键词
弓形虫/焦磷酸酶/酸性钙体/多克隆抗体Key words
Toxoplasma gondii/vacuolar proton pyrophosphatase/acidocalsisome/polyclonal antibodies引用本文复制引用
佟澄碧,郝文波,罗树红,肖斌,程莎莎,廖小青,潘迪..兔抗弓形虫Ⅰ型液泡型质子焦磷酸酶多克隆抗体的制备及鉴定[J].南方医科大学学报,2015,35(8):1137-1142,6.基金项目
国家自然科学基金(81171608) Supported by National Natural Science Foundation of China (81171608). （81171608）
