南方医科大学学报2015，Vol.35Issue(8)：1170-1174,5.
Wnt7a-Akt/mTOR信号通路在肾衰营养胶囊改善慢性肾衰竭大鼠骨骼肌萎缩中的作用
Shenshuai Yingyang capsule ameliorates muscle atrophy in rats with chronic renal failure:role of Wnt7a-Akt/mTOR signal pathway
摘要
Abstract
Objective To observe the effect of Shenshuai Yingyang Capsule (SSYYJN) in ameliorating muscle atrophy in rats with chronic renal failure (CRF) and explore the role of Wnt7a-Akt/mTOR signal pathway in mediating this effect. Methods Male rats were randomly assigned to 5/6 nephrectomy group and sham-operated group, and the former group was further randomly divided into CRF model group, KA group, and SSYYJN group. The size of anterior tibia muscle was examined microscopically with HE staining. Protein synthesis in the soleus muscle was investigated by 14C-phenylalanine experiment, and the expression of Wnt7a, frizzled-7, phospho-Akt, phospho-mTOR and GAPDH were detected with Western blotting. Results The body weight, the wet and dry weight, cross-sectional area, and muscle protein synthesis of the anterior tibia muscles, and expressions of the proteins in the Wnt7a/Akt signaling pathway all increased significantly in SSYYJN and KA groups as compared with those in the model group. Conclusion SSYYJN can effectively improve muscle atrophy in the rat model of CRF possibly by reversing the reduction in the expressions of Wnt7a/Akt signaling pathway proteins in the skeletal muscles.关键词
慢性肾衰竭/营养不良/骨骼肌萎缩/Wnt7a/肾衰营养胶囊Key words
chronic renal failure/malnutrition/muscle atrophy/Wnt7a/Shenshuai Yingyang Capsule引用本文复制引用
王明,汪东涛,尹懿,鲁路,石莹,黄燕峰,陈德秀,魏连波..Wnt7a-Akt/mTOR信号通路在肾衰营养胶囊改善慢性肾衰竭大鼠骨骼肌萎缩中的作用[J].南方医科大学学报,2015,35(8):1170-1174,5.基金项目
国家自然科学基金(81173457,81373808,81403215) Supported by National Natural Science Foundation of China (81173457, 81373808, 81403215). （81173457,81373808,81403215）
