中国妇幼健康研究2015，Vol.26Issue(4)：672-675,4.DOI:10.3969/j.issn.1673-5293.2015.04.006
Notch1 蛋白在儿童急性白血病中的表达及意义
Expression of Notch1 protein in children with acute leukemia and its significance
赵晓莉 1钱新宏 2潘凯丽 2李迎侠 2王英娟2
作者信息
- 1. 陕西省第四人民医院儿科,陕西西安710043
- 2. 第四军医大学西京医院儿科,陕西西安710032
- 折叠
摘要
Abstract
Objective To study the expression of Notch1 protein in children with acute leukemia ( AL) and to explore the relationship of Notch1 expression with immunophenotype.Methods The bone marrow smears of 42 cases of AL and 22 healthy children or cases of nonmalignant hematologic disease were collected during October 2009 to November 2011.Immunocytochemical assay was used to detect the expression of Notch1 protein in the cases.Results Of 42 cases of AL, there were 32 cases of acute lymphocytic leukemia ( ALL) including 25 cases of B-ALL and 7 cases of T-ALL, and 10 cases of acute myelogenous leukemia ( AML) .The expression rate of Notch1 protein was 60.0%(6/10), 71.4%(5/7), 28.0%(7/25)and 13.6%(3/22)in AML group, T-ALL group, B-ALL group and the control group, respectively.Compared with the control group, the expression rate of Notch1 protein in AML group and T-ALL group was remarkably increased, and the differences were significant (χ2 value was 7.311 and 8.878, respectively, both P<0.05).The expression rate of Notch1 protein was higher in B-ALL group than in the control group, but the difference was not significant (P>0.05).The difference in the expression of Notch1 protein between T-ALL group and B-ALL group was not significant (χ2 =4.400,P<0.05) .The expression rate of Notch1 protein positive cells increased gradually in ALL-LR group, ALL-MR group and ALL-HR group, and that was higher in HR group than in LR group and MR group.But the comparison between groups showed no significant difference (P>0.05).Conclusion There are significant differences in the Notch1 protein among children with different types of AL, and abnormal expression of Notch1 is correlated with the occurrence of T-ALL.Notch1 signals might play important role in AL, which needs further researches to confirm.关键词
急性白血病/Notch1蛋白/免疫细胞化学/儿童Key words
acute leukemia ( AL)/Notch1 protein/immunocytochemistry/children分类
医药卫生引用本文复制引用
赵晓莉,钱新宏,潘凯丽,李迎侠,王英娟..Notch1 蛋白在儿童急性白血病中的表达及意义[J].中国妇幼健康研究,2015,26(4):672-675,4.
