华北农学报Issue(4)：31-34,4.DOI:10.7668/hbnxb.2015.04.006
萼脊兰 TUB 基因片段的克隆及序列分析
Cloning and Sequence Analysis of TUB Gene Fragment from Sedirea japonica
摘要
Abstract
TUB of Sedirea japonica was cloned and sequence analyzed,and the result would provide an impor-tant basis for selecting a reference gene and studying the function of TUB.The total RNA of Sedirea japonica scapes was extracted by CTAB method and the partial sequence of Sedirea japonica TUB was cloned by RT-PCR,which was 1 055 bp long and encoded 351 amino acids residues.Its amino acid sequence contained a conservative region GGGTGSG.The sequencing result revealed that the TUB gene fragment from Sedirea japonica homology comparison with other plants TUB gene sequences in the GenBank shows that they share 75%-96% nucleotide sequence ho-mology,96% nucleotide sequence homology with Doritaenopsis hybrid TUB,the amino acid sequence homology is a-bove 96% and amino acid sequence homology high similarity with the monocotyledon.The cloned genes were TUB gene fragment,which were registered them into GenBank(respective accession number:KP686397).关键词
萼脊兰/TUB 基因/克隆/序列分析Key words
Sedirea japonica/TUB gene/Cloning/Sequence analysis分类
生物科学引用本文复制引用
蒋素华,梁芳,王洁琼,李艳辉,王默霏,崔波..萼脊兰 TUB 基因片段的克隆及序列分析[J].华北农学报,2015,(4):31-34,4.基金项目
河南省科技攻关项目（092102110128）；郑州市科技计划项目（112PPTGY250-3）；校级项目 （）
