华北农学报Issue(4)：35-42,8.DOI:10.7668/hbnxb.2015.04.007
羊草脂氧合酶同源蛋白基因 LcLHP 的克隆与表达分析
Cloning and Expression of Lipoxygenase Homology Protein from Leymus chinensis
摘要
Abstract
In order to know the relationships between Lipoxygenase and salt stress,a full length cDNA named LcLHP related to saline-alkali tolerance was cloned from Leymus chinensis by RT-PCR and RACE(Rapid amplifica-tion of cDNA ends).LcLHP gene (GenBank:KJ472894)with 537 bp open reading frame encoded a protein with a predicted length of 1 78 amino acids.LcLHP has PLAT /LH2 structural domain,which is induced by stress.Homolo-gy analysis showed that the deduced amino acid sequence of LcLHP shared high homology with PLAT /LH2 protein family of Spartina alterniflora and PLAT stress protein.Protein function prediction showed N terminal of LcLHP may have signal peptide,act as enzyme.The expression of LcLHP was analyzed by RT-PCR under salinity-alkalinity stressed condition.With the extension of the salinity-alkalinity stressed time,the expression of LcLHP increased from 0 to 1 2 h in the leaf,and then decreased,the peak of expression level arrived at 1 2 h.Construction of pGEX-LcLHP ex-pression vector,IPTG induced prokaryotic expression,we got GST-LcLHP fusion protein and verified by Western Blot.关键词
羊草/盐碱胁迫/LcLHP/RACE 克隆/表达分析Key words
Leymus chinensis/Saline-alkali stress/LcLHP/RACE cloning/Expression analysis分类
生物科学引用本文复制引用
张旸,孙天旭,郭勃予,汤明威,李玉花,解莉楠..羊草脂氧合酶同源蛋白基因 LcLHP 的克隆与表达分析[J].华北农学报,2015,(4):35-42,8.基金项目
“十二五”农村领域国家科技计划课题项目 （）
