华北农学报Issue(4)：95-100,6.DOI:10.7668/hbnxb.2015.04.017
高表达转 C4型 PEPC 基因水稻在低氮下诱导碳氮酶稳定光合作用
Transgenic Rice with High Expression of C4-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions
摘要
Abstract
The objective of this study was to reveal transgenic rice with high expression of maize C 4-PEPC pho-tosynthetic performance under low nitrogen conditions,two rice genotypes including C 4-PEPC transgenic rice (PC) and untransgenic Kitaake rice(WT)in the pot experiments were chosen to measure their photosynthesis characteris-tics,SPAD values,PEPC,Rubisco,NR and GS activities of flag leaves at different flowering stage under different ni-trogen applications(normal nitrogen 300 kg /ha,low nitrogen 65 kg /ha)and their yield component were investigated after harvest.The results showed that the net photosynthetic rate of PC compared with WT were increased by 1 3.1 0% (P <0.05 )and 29.29% (P <0.05 ),respectively in the 1 4,28 d after flowering under low nitrogen treatment.At the same time,under low concentrations of nitrogen,relative to untransformed wild type rice,activity of Rubisco carboxylase of PC were increased by 67.86% and 52.63% (P <0.05)in the 1 4,28 d after flowering re-spectively,while activity of nitrate reductase of PC were increased 79.49% (P <0.05)and 1 7.96%.Meanwhile, activity of glutamine synthetase of PC was increased 28.48% (P <0.05)only in the 28 days after flowering com-paring with WT.But we did not find significant differences in the yield of PC and WT under low nitrogen condi-tions.Therefore,PC maintain high net photosynthetic rate by inducing the activity of carbon and nitrogen key en-zyme improvement under low nitrogen conditions.关键词
高表达转 C4 型 PEPC 基因水稻/低氮/光合特性Key words
High expression of maize C 4-PEPC transgenic rice/Low nitrogen/Photosynthesis分类
农业科技引用本文复制引用
唐玉婷,李霞,陆巍,魏晓东..高表达转 C4型 PEPC 基因水稻在低氮下诱导碳氮酶稳定光合作用[J].华北农学报,2015,(4):95-100,6.基金项目
国家自然科学基金项目（No．31371554）；江苏省自主创新基金项目（CX［（14）5004］）；江苏省自然科学基金项目 （）
