黑龙江农业科学Issue(8)：115-118,119,5.DOI:10.11942/j.issn1002-2767.2015.08.0115
响应面法优化碱溶性龙胆多糖的提取研究
Optimization Extraction of Alkali-solubility Polysaccharide from Gentiana scabra Bunge by Response Surface Methodology
温钢 1冯凯1
作者信息
- 1. 吉林化工学院，化工与生物技术学院，吉林吉林132022
- 折叠
摘要
Abstract
For the further development of the alkali‐soluble polysaccharide extraction ,response surface method‐ology (RSM) was used to optimize the extraction conditions of alkaline solubility polysaccharides from the Gentiana scabra Bunge .The method of alkali extraction and alcohol sink was used to extract polysaccharides . The effect of extraction times ,extraction temperature ,extraction time ,ratio of water to raw material ,and NaOH concentration on the yield of Gentiana scabra Bunge polysaccharides were investigated .The results showed that combining single factor test and response surface methodology ,the optimum extraction conditions were chosen as follows:extraction temperature was 74℃ ,ratio of water to raw material was 1∶25 ,extraction time was 1 .8 h ,one time extraction and the NaOH concentration was 9% .Under the optimize conditions ,the GSP predicted value of yield was 4 .4% ,and the GSP experimental yield was 4 .32 ± 0 .124% ,the results were reasonable with expectation .关键词
多糖/优化/龙胆多糖/碱溶性Key words
polysaccharides/optimization/Gentiana scabra Bunge/alkali-solubility分类
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温钢,冯凯..响应面法优化碱溶性龙胆多糖的提取研究[J].黑龙江农业科学,2015,(8):115-118,119,5.
