黑龙江农业科学Issue(9)：64-68,69,6.DOI:10.11942/j.issn1002-2767.2015.09.0064
13株植物病原菌拮抗细菌的筛选
Screening of Antagonistic Bacterium Against 13 Kinds of Common Plant Pathogenic Fungi
摘要
Abstract
In order to obtain bio‐control bacteria with highly effective and broad spectrum antagonism effect a‐gainst plant pathogenic fungi ,282 isolates were obtained by the method of dilution coated tablet and streak plate from lab and roots and rhizosphere soil samples of grapes ,tomatoes and cucumbers .With 13 kinds of common plant pathogenic fungi as indicators ,inhibitory effect of fermentation liquid and fermentation filtrate a‐gainst the indicators were tested by the methods of tablet confrontation and agar disk diffusion .The results showed that 9 bacterial strains had efficient and stable inhibitory activity ,and 4 strains of them fermentation liquid had efficient inhibitory activity against all the indicators ,showing broad antimicrobial spectrum against pathogenic fungi .Among them ,the inhibition effect of k8j was significantly higher than other isolates ,and it had the highest inhibition effect against Setosphaeria turcica ,with the inhibition rate was 92 .02% .9 bacterial strains fermentation filtrate also showed some inhibitory effect .HMGR‐2 had the best inhibition effect against Setosphaeria turcica with the inhibition zone diameters was 4 .15 cm .Fermentation filtrate of k8j had good in‐hibition effect against 11 indicators (except for Pyricularia grisea and Botrytis cinerea) ,and compared with other strains ,its inhibitory action reached the significant level ,and it showed that metabolites also had broad spectrum of antibacterial effect .关键词
植物病原真菌/拮抗细菌/发酵液/发酵滤液/拮抗性Key words
plant pathogenic fungi/antagonistic bacterium/fermentation liquid/fermentation filtrate/antago-nistic activity分类
农业科技引用本文复制引用
王爽,李新民,刘春来,杨帆,夏吉星,王克勤,刘兴龙..13株植物病原菌拮抗细菌的筛选[J].黑龙江农业科学,2015,(9):64-68,69,6.基金项目
国家高技术研究发展计划（863计划）资助项目（2011AA10A205）；黑龙江省农业科技创新工程资助项目 （）
