激光生物学报Issue(4)：373-376,399,5.DOI:10.3969/j.issn.1007-7146.2015.04.013
苏云金芽胞杆菌6618杀虫菌株的分离和鉴定
Isolation and Characterization of an Insecticidal Bacillus thuringiensis Strain 6618
摘要
Abstract
118 soil samples were collected from different parts of our country and a new Bacillus thuringiensis strain 6618 was isolated by temperature screening method. This strain could produce typical bipyramidal crystal under micro-scope observation. PCR analysis showed that it contained cry1-type gene. SDS-PAGE analysis indicated that this strain expressed a major protein band about 130 kD, which was verified by mass spectrometry analysis to be the products of Cry1Ae and Cry1Ac protoxins. Sequence analysis showed that the cry1Ac gene in this strain was identical to the pub-lished cry1Ac1 gene, while the cry1Ae gene was found to be different from the known gene. The crystal-spore mixture of the strain 6618 exhibited high toxicity towards the larvae of Helicoverpa armigera. The isolated Bacillus thuringiensis strain 6618 with high toxicity will not only enrich the reserve of Bacillus thuringiensis strains of our country, but also pro-vide resource for the development of new efficient biological pesticide.关键词
苏云金芽胞杆菌/菌株筛选/cry基因/质谱分析/杀虫活性Key words
Bacillus thuringiensis/strain screening/cry gene/mass spectrometry/insecticidal activity分类
生物科学引用本文复制引用
张晨,黄菲,陆秀青,谢俊雁,丁学知,夏立秋,孙运军..苏云金芽胞杆菌6618杀虫菌株的分离和鉴定[J].激光生物学报,2015,(4):373-376,399,5.基金项目
湖南省教育厅高校创新平台开放基金项目(14K058) （14K058）
湖南师范大学青年优秀人才培养计划项目(ET13105) （ET13105）
