激光生物学报Issue(4)：377-381,5.DOI:10.3969/j.issn.1007-7146.2015.04.014
丹参愈伤组织培养中激素浓度的优化研究
Study on Optimization of Hormone Concentration in Callus Culture of Saliae Miltiorrhiza Bge
摘要
Abstract
Objective: To seek optimum combination of hormone concentration during callus subculture of Glycyrrhiza uralensis. Methods: The seed of Saliae Miltiorrhiza Bge as explants, first generation callus induced by embryo root and hypocotyledonary axis of sterilized seed as material, the net fresh weight of callus as indicator, mathematical model were established by quadratic orthogonal rotary composite experimental design. The concentration of hormones were optimized by method of response surface. Result: The optimum combination of hormone concentration during callus subculture of Saliae Miltiorrhiza Bge is 6-BA 1. 514 mg/L, NAA 2. 059 mg/L, 2,4-D 2. 137 mg/L. The net fresh weight of callus is 35. 66g.关键词
丹参/响应面法/愈伤组织/激素浓度Key words
Saliae Miltiorrhiza Bge/method of response surface/callus/hormone concentration分类
农业科技引用本文复制引用
荆瑞勇,王丽艳,郭永霞..丹参愈伤组织培养中激素浓度的优化研究[J].激光生物学报,2015,(4):377-381,5.基金项目
黑龙江省自然科学基金(QC2012C049) （QC2012C049）
