吉林中医药Issue(9)：934-937,4.DOI:10.13463/j.cnki.jlzyy.2015.09.021
逍遥散对慢性束缚应激所致肝郁脾虚证大鼠饥饿素的影响
Xiao Yao San on Ghrelin levels in rats with liver depression and spleen deficiency syndrome indeced by immobilization stress
摘要
Abstract
Objective This experiment was designed to measure the effect of Xiao Y ao San on treating liver depression and spleen deficiency syndrome by recording the changes of Ghrelin levels in the blood and the stomach bottom of fundic glands in chronic immobilization stress (CIS ) model rats .Methods The 24 male SD rats were randomly divided into 3 groups (8 rats for each group):normal group ,model group and Xiao Yao San group .We didn’t make any deal with normal group ,but model and Xiao Yao San groups by continuous 21 day immobilization stress cause induced liver depression and spleen defi-ciency syndrome ,before every morning bondage administered Xiao Yao San (3 .854 g/Kg・d) to the Xiao Yao San group and administered a saline liquid (2 mL ) to the normal and model groups .We used ELISA to measure the Ghrelin levels in both the blood and stomach bottom after death on day 22 .Result After 21 days ,the model group rat body weight ,food in-take ,water quantity increase slower than normal group .The Xiao Yao San group increase faster than model group ,they have a statistical significance ( P<0 .05 or P<0 .01 ) .The model group showed a significant decrease in the Ghrelin level con-tents in both the blood and stomach bottom compared to the Xiao Y ao San and the normal groups ( P<0 .05 ) ,but the Xiao Yao San group had a higher level of Ghrelin in stomach bottom than model group ( P<0 .05) .Normal groups had similar Ghrelin level contents in stomach bottom;The model group had a lower level of Ghrelin in the blood than Xiao Y ao San group ( P<0 .01) .Conclusion Xiao Yao San can effectively treat rats with immobilization stress induced liver depression and spleen deficiency condition by raising the levels of Ghrelin in both the blood and stomach bottom .关键词
慢性束缚应激/肝郁脾虚/逍遥散/饥饿素Key words
chronic immobilization stress/liver depression and spleen deficiency/Xiao Yao San/Ghrelin分类
医药卫生引用本文复制引用
金钟晔,王少贤,白明华,李娜,景玉霞,刘群,陈家旭..逍遥散对慢性束缚应激所致肝郁脾虚证大鼠饥饿素的影响[J].吉林中医药,2015,(9):934-937,4.基金项目
国家自然科学基金项目（30672578）；国家杰出青年科学基金课题（30825046）。 （）
