检验医学与临床Issue(17)：2548-2550,2553,4.DOI:10.3969/j.issn.1672-9455.2015.17.026
液相基因芯片技术在崇明地区人乳头瘤病毒检测和分型中的应用
Application of liquid gene chip technology in human papillomavirus detection and typing in Chongming area
摘要
Abstract
Objective To explore the application of the liquid gene chip technology in the human papillomavir‐us (HPV) detection and genotyping in Chongming area .Methods 500 cases of cervical exfoliated cells in the gyne‐cology outpatient department of our hospital from May 2011 to April 2013 were selected and performed the HPV genotyping by using the liquid gene chip technology .80 cases were performed the pathological diagnosis and divided into the groups of inflammation reaction ,normal cytology ,cervical cancer ,cervical intraepithelial neoplasia(CIN)Ⅰ , CINⅡ ,CINⅠ - Ⅱand CINⅢ according to the histopathology .The pathological diagnosis results and subtype distri‐bution results of HPV DNA were combined to conduct the analysis .The relationship between the degree of cervical lesions and HPV infection genotypes in Chongming area was analyzed .Results Among 500 specimens ,180 cases were HPV‐positive ,the HPV total infection rate was 36% ,22 genotypes were detected ,according to the descending order which were in turn HPV‐16 ,HPV‐52 ,HPV‐58 ,HPV‐18 ,HPV‐11 ,HPV‐6 and HPV‐31 .Among them ,the high risk infection accounted for 85 .96% and low‐risk infection for 14 .04% .Among 500 cases of samples ,180 cases were HPV‐positive patients ,including 44 patients(24 .44% ) aged 21 -25 years old ,11 cases(6 .15% ) patients aged 51-67 years old ,with age increasing ,number of HPV‐positive cases was decreased .With the increase of cervical le‐sions severity ,the HPV infection rate was increased ,the pairwise comparison showed that the differences between the inflammatory group and the normal group ,between CINⅢ and CINⅡ ,between cancer group and CINⅡ group ,be‐tween cervical cancer group and the CINⅢ group was not statistically significant (P>0 .05);the differences of pair‐wise comparison in remaining 11 pairs had statistically significant difference(P<0 .05) .With the increase of cervical lesions ,the multiple HPV infection rate was gradually increased ,but only the differences between the inflammation group with the CINⅢ ,CINⅡ groups were statistically significant(P<0 .05) .With the histopathology as the diag‐nostic standard ,in testing CINⅢ and CINⅡ by the liquid chip HPV DNA ,the specificity was 76 .63% ,the sensitivi‐ty was 88 .57% ,negative predictive value was 92 .16% and the positive predictive value was 68 .89% .Conclusion Common genotypes of HPV infection in Chongming area are HPV‐16 ,HPV‐52 ,HPV‐58 ,HPV‐18 ,HPV‐11 ,HPV‐6 and HPV‐31 .The HPV‐positive is more common in young women ,and the HPV multiple infection and infection rate are aggravated with the aggravation of cervical lesion severity .Thus the liquid chip HPV DNA examination has a very important value for large‐scale screening and clinical diagnosis of cervical lesions .关键词
液相基因芯片技术/人乳头瘤病毒/检测/分型Key words
liquid gene chip technology/human papillomavirus/testing/typing引用本文复制引用
张莉,冷俊,周晔,张蕾,杨惠萍,陈峰..液相基因芯片技术在崇明地区人乳头瘤病毒检测和分型中的应用[J].检验医学与临床,2015,(17):2548-2550,2553,4.基金项目
上海市崇明县科学技术发展资金资助项目（CK2011-28）。 （）
