检验医学与临床Issue(17)：2582-2583,2586,3.DOI:10.3969/j.issn.1672-9455.2015.17.039
H ER-2基因表达在乳腺原发癌和淋巴结转移癌发病中的作用研究
Role of HER-2 gene expression in pathogensis of breast primary carcinoma and lymph node metastatic carcinoma
欧武英 1钟威达2
作者信息
- 1. 海南省海口市人民医院检验科 570208
- 2. 海南妇产科医院检验科，海口 570105
- 折叠
摘要
Abstract
Objective To investigate the expression and significance of HER‐2 gene expression in primary breast cancer and lymph node metastatic carcinoma by using fluorescence in situ hybridization technique .Methods 40 cases of primary breast cancer in the Haikou Municipal People′s Hospital from January 2013 to January 2014 were selected as the research subjects and their clinical data were retrospectively analyzed .The fluorescence in situ hybrid‐ization (FISH) technique and immunohistochemical (IHC) techniques were used to detect the expression of HER‐2 gene in operation excision specimens of all patients .Results The positive rate of FISH was 42 .5% ,which of IHC was 57 .5% ;in negative IHC ,the coincidence rate with FISH was 88 .2% ,the coincidence rate of IHC (+ ) with FISH was 37 .5% ,which of IHC (+ + ) with FISH was 83 .3% and which of IHC (+ + + ) with FISH was 100% . The lymph node metastasis positive rate was 66 .67% ,non‐lymph node metastasis positive rate was 32 .14% ,the difference was statistically significant(P<0 .05) .Conclusion Detecting HER‐2 gene by IHC ,if the result is negative or strongly positive ,the coincidence rate with FISH is higher ,at the same time the HER‐2 gene expression is posi‐tivele correlated with axillary lymph node metastasis .关键词
荧光原位杂交技术/乳腺原发癌/淋巴结转移癌/HER-2基因Key words
fluorescence in situ hybridization/primary breast cancer/lymph node metastasis/HER-2 gene引用本文复制引用
欧武英,钟威达..H ER-2基因表达在乳腺原发癌和淋巴结转移癌发病中的作用研究[J].检验医学与临床,2015,(17):2582-2583,2586,3.
