检验医学与临床Issue(18)：2647-2649,2653,4.DOI:10.3969/j.issn.1672-9455.2015.18.001
血培养实验室污染菌群分布与阳性报警时间的判断
Laboratory contamination bacterial distribution of blood culture and judgment of positive alarm time
摘要
Abstract
Objective To understand the bacterial contamination situation of blood culture to provide the labo‐ratory evidence and experience for the clinical judgment of blood culture contamination by retrospectively analyzing the pathogenic bacterial detection situation of blood culture and laboratory judgment detection of contamination bacte‐ria in the First Affiliated Hospital of Xi′an First Affiliated Hospital of Xi′an Jiaotong University Jiaotong University . Methods The blood culture samples collected in the First Affiliated Hospital of Xi′an Jiaotong University from Jan‐uary 2014 to December 2014 were retrospectively analyzed .The comprehensive analysis was performed according to the laboratory assessment scheme on blood culture contamination and combining with the bacterial species and posi‐tive report time for judging the blood culture contamination and analyzing the bacterial species and their composition . Results This research contained 17 941 bottles of blood culture ,including 7 193 sets of negative and 837 sets of posi‐tive ,the complete set rate was 89 .5% .The positive rate of blood culture was 10 .4% ,of which the contamination rate was 1 .6% .The main contaminated bacteria were coagulase negative staphylococcus ,and average alarm time of con‐taminated bacteria in aerobic and anaerobic bottles was 1 .34 ,1 .29 d ,respectively .The pathogenic bacteria and oppor‐tunistic pathogenic bacteria were common in the culture results of two bottles with the inconsistent culture results . Conclusion Blood culture contaminating bacteria are mainly skin resident bacteria .To reduce the contamination rate of blood culture ,it is vital to strictly execute the standard blood sampling procedure ,and emphasize the sterilization of blood‐collecting environment in hospitalized patients .关键词
血培养/污染/细菌Key words
blood culture/contamination/bacteria引用本文复制引用
答嵘,吴友伟,王伟,雷金娥..血培养实验室污染菌群分布与阳性报警时间的判断[J].检验医学与临床,2015,(18):2647-2649,2653,4.基金项目
国家自然科学基金资助项目（81000767）。 （）
