临床口腔医学杂志Issue(9)：521-525,526,6.DOI:10.3969/j.issn.1003-1634.2015.09.004
碱性成纤维细胞生长因子联合SonicHedgehog时序调控骨髓间充质干细胞的体外实验研究
In vitro study of regulating bone marrow derived mesenchymal stem cells with sequential delivery of basic fi-broblast growth factor and sonic hedgehog
摘要
Abstract
Objective:The aim of this study was to delivery basic fibroblast growth factor(bFGF) and sonic hedgehog (Shh) to bone marrow derived mesenchymal stem cells (BMSCs),and then to detect the effect for osteogenic marker genes. Method:bFGF and Shh were constructed to the recombinant adeno-associated virus,respectively(rAAV2-tet-off-bFGF and rAAV2-Shh). The previous viral vector allowed for regulation of the bFGF expression by the addition of doxycycline,a te-tracycline analogue. Several osteogenic markers were detected by quantitative real-time reverse transcriptase polymerase chain reaction and western blot. Result:BMSCs were constructed successfully by two recombinant virus that were regulated by Dox. The expression of osteogenic marker mRNA and protein had an increased tendency after two genes transduction(P<0.05). Conclusion:Sequential delivery of angiogenic and osteogenic factors likely has a synergistic effect,which could not only enhance the cell proliferation,but also improve the expression of osteogenic marker of BMSCs.关键词
碱性成纤维细胞生长因子/Sonic Hedgehog/骨髓间充质干细胞/基因传递Key words
basic fibroblast growth factor/sonic hedgehog/bone marrow derived mesenchymal stem cells/gene de-livery分类
生物科学引用本文复制引用
宋珂,陈美玲,石琦,青莹,曹颖光..碱性成纤维细胞生长因子联合SonicHedgehog时序调控骨髓间充质干细胞的体外实验研究[J].临床口腔医学杂志,2015,(9):521-525,526,6.基金项目
国家自然科学基金（81400490）湖北省自然科学基金 （）
