临床神经病学杂志Issue(4)：281-283,3.
JAK2／STAT3信号转导通路在大鼠脑缺血再灌注损伤中的作用
The effect of JAK2/STAT3 signaling pathway in rat with cerebral ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the effect of JAK2/STAT3 signaling pathway in rat with cerebral ischemia-reperfusion injury.Methods Fifty-four SD rats were randomly divided into sham group, ischemia-reperfusion ( I/R) group and JAK2/STAT3 pathway inhibitor group (AG group), and there were 18 rats in each group.The middle cerebral artery cerebral ischemia/reperfusion model was builded by modified thread embolism method.AG490 ( 1 mg/kg) were given to the rat of AG group at 5 min before reperfusion.Neurological deficit score was assessed after model building.The infarct volume was measured by TTC staining.The changes of brain cell apoptosis was measured by terminal-deoxynucleoitidyl transferase mediated nick end labeling.The expression of caspase-3 and phosphorylated STAT3 ( p-STAT3) were detected by immunohistochemistry and Western blot respectively.Results Compared with those in I/R group, the neurological deficit score, infarct volume and apoptotic cell number in AG group were significantly decreased ( all P<0.05 ) .Compared with those in I/R group, the positive cell of caspase-3 and p-STAT3 expression in sham group and AG group were significantly decreased ( all P<0.05 ) .The positive cell of caspase-3 in sham group was significantly decreased than that in AG group ( P<0.05 ) .Conclusion JAK2/STAT3 signaling pathway is involved in the rat with cerebral ischemia-reperfusion injury, inhibiting JAK2/STAT3 signaling pathway can reduce the expression of caspase-3 and neuronal apoptosis after ischemia-reperfusion injury, and it can improve neurological deficit symptoms.关键词
缺血再灌注/JAK2/STAT3/细胞凋亡/caspase-3Key words
ischemia-reperfusion/JAK2/STAT3/apoptosis/caspase-3分类
医药卫生引用本文复制引用
韦家俊,廖小明,王耀辉,刘开祥..JAK2／STAT3信号转导通路在大鼠脑缺血再灌注损伤中的作用[J].临床神经病学杂志,2015,(4):281-283,3.基金项目
桂林市科技开发项目（0110119－1－6）；广西省自然科技基金 （）
