林业科学Issue(8)：67-73,7.DOI:10.11707/j.1001-7488.20150809
2种生防菌株对杨树溃疡病原葡萄座腔菌的抑制作用
Inhibitory Effects of Two Biocontrol Fungous Strains on Poplar Canker Botryosphaeria dothdea
摘要
Abstract
Objective]Poplar canker is one of the major biological disaster of poplar plantations in China. Techniques of biological control to restrain poplar canker are sustainable and effective. Trichoderma aureoviride strain YGF9 and Fusarium equiseti strain LX6F2 are two biocontrol strains which were isolated from poplar tissue and soil,respectively. They may have the potential to prevent from and control poplar canker. In order to explore the mechanism of these strains in controlling disease, biocontrol efficacy of the strain YGF9 and the strain LX6F2 on poplar canker caused by Botryosphaeria dothidea were investigated with its antagonism enzyme and inhibition of pathogen’s growth. [Method]Production of chitinase,cellulose enzyme,β-1,3-glucanase and protease by the two strains was determined through the reaction and growth status of the two biocontrol strains grown on chitin medium,cellulose medium,β-1,3-glucan culture medium and casein medium. Effects of the two biocontrol strains on the pathogen hyphal growth and spore germination were observed through a microscope. [Result]The results showed that the strains YGF9 and LX6F2 could make β-1,3 glucanase medium’s blue color disappear in metabolic process,indicating that the two strains could produce β-1,3-dextranase. The two strains had the ability to form hydrolyzed circle on casein medium,suggesting that the two strains had the ability to produce protease; The hydrolyzed circle and transparent circle were not generated by the two strains in chitin medium and cellulose hydrolysis medium,inferring that the two strains were not able to produce chitinase and cellulase. The strain LX6F2 hypha grew parallelly to pathogen hyphae,winded or interspersed with pathogen hyphae,so as to make the pathogen hyphae distortion,deformation and enlargement abnormal. The strain YGF9 hypha made pathogen hyphae folding,deformity,fracture,thereby preventing pathogen hyphal growth. The fermentation products of the two biocontrol strains could lead to spores of poplar canker pathogen B. dothidea deformity,and change in the spore internal structure, thus the pathogen spores couldn’t germinated normally. The antifungous substances could make germ tubes shorter and twist deformity,so that germ tubes couldn’t stretch forward. The inhibition rate of the biocontrol strains to pathogenic spore germination could reach to more than 94%.[Conclusion]It was found that the two biocontrol strains could produce antagonism enzymes and inhibit pathogen’s growth,which can interpret why the strain YGF9 and strain LX6F2 could prevent from poplar canker. Chitinase and cellulase produced by the two biocontrol strains were not found in the present experiment,for which,the further study is required by using different experimental methods for validation. In addition, the present study only studied the ability of the two biocontrol strains producing antagonism enzyme in qualitative analysis, the amount of enzyme production,the separation and application of these antagonism enzymes,as well as quantitative analysis of the amount should be studied later.关键词
杨树溃疡病/黄绿木霉/木贼镰刀菌/葡萄座腔菌/生防菌株/抑菌效果Key words
poplar canker/Trichoderma aureoviride/Fusarium equiseti/Botryosphaeria dothidea/biocontrol strains/inhibitory effect分类
生物科学引用本文复制引用
杨蕾,周国英,梁军..2种生防菌株对杨树溃疡病原葡萄座腔菌的抑制作用[J].林业科学,2015,(8):67-73,7.基金项目
国家“十二五”科技支撑计划课题“商品林重大病虫害监测预警与防控技术研究示范”(2012BAD19B08) （2012BAD19B08）
湖南省研究生科研创新项目“杨树溃疡病生防菌研究”(CX2014B328) （CX2014B328）
林业公益性行业科研专项“重大森林病虫灾害防控技术的关键理论基础”(201204501)。 （201204501）
