山东中医药大学学报Issue(5)：472-475,4.
蟾毒灵胃肠道吸收动力学研究
Study on Kinetics of Gastrointestinal Absorption of Bufalin
摘要
Abstract
Objective:To explore the absorption kinetics and absorption mechanisms of bufalin in stomach , intestine and gastrointestine in rats,so as to provide a basis for the development of bufalin. Methods:The absorption kinetics of gastrointestine in rats were obtained by in situ perfusion method and the concentration was determined by HPLC method. Results:3 hours after perfusion,bufalin in different concentrations (10.0, 20.0,30.0μg·mL-1)can be absorbed in the stomach and the cumulative uptake rate was around 90%. The absorption was not rated to the concentration of the drug. The absorption in the small intestine showed the first-order kinetics characteristics and the absorption rate constant of the three concentrations were 0.017 , 0.017 and 0.019/cm·h. Bufalin was absorbed at all segments of the intestine and it was a first-order pro-cess. The absorption rate constant in the duodenum,jejunum,ileum and colon were 0.024,0.027,0.022 and 0.021/cm·h-1. Conclusion:Bufalin can be absorbed at stomach and all segments of intestine of rats. It was a first-order process with passive diffusion mechanism.关键词
蟾毒灵/吸收动力学/高效液相色谱法/大鼠Key words
bufalin/absorption kinetics/HPLC/rat分类
医药卫生引用本文复制引用
赵杰,胡乃合,战兵,林桂涛..蟾毒灵胃肠道吸收动力学研究[J].山东中医药大学学报,2015,(5):472-475,4.基金项目
济南市科技局高校自主创新项目 （）
