山东医药Issue(32)：5-7,3.DOI:10.3969/j.issn.1002-266X.2015.32.002
食管鳞状细胞癌组织 P16基因启动子区CpG 单位甲基化状态检测及意义
Detection and significance of CpG unit methylation in P16 gene promoter region of esophageal squamous cell carcinoma
摘要
Abstract
Objective To detect the CpG unit methylation in the P 16 gene promoter region of esophageal squamous cell carcinoma tissues and the significance .Methods We collected 35 cases of esophageal squamous cell carcinoma sam-ples ( observation group ) and 46 cases of normal esophageal tissues ( control group ) .We used MassARRAY methylation DNA quantitative analysis technology to detect the methylation status of 19 CpG units of P16 gene promoter region , and we used Rank test to compare the CpG methylation rates of the two groups .Results The 19 CpG unit methylation rates in the P16 gene promoter region of the observation group and control group were 9.06%±7.11%and 8.48%±6.34%, respective-ly.No significant difference was found between the two groups , P>0.05.The methylation rate of P16 gene in ESCC of CpG unit 11-12 was 11.34%±8.47%in the observation group, and 7.25%±5.60%in the control group (P<0.05);and no significance was found in the methylation rate of other 18 units between the two groups ( all P>0.05 ) .Conclusion The methylation of CpG11-12 site in the P16 gene promoter region may be associated with the esophageal squamous cell carcinoma .关键词
食管鳞状细胞癌/P16基因/CpG单位/甲基化Key words
esophageal squamous cell carcinoma/P16 gene/CpG unit/methylation分类
医药卫生引用本文复制引用
刘芳,康雪,刘晓燕,张宁,齐翠花,黎永军,郑勇,陈卫刚..食管鳞状细胞癌组织 P16基因启动子区CpG 单位甲基化状态检测及意义[J].山东医药,2015,(32):5-7,3.基金项目
国家自然科学基金资助项目（81260362）；石河子大学医学院基础与临床医学联合科研基金资助项目（LHJJ2010B04）。 （）
