摘要
Abstract
Objective To observe the influence of bone marrow mesenchymal stem cells ( BMSCs)-derived exosome on the proliferation and invasion of mouse breast cancer cells 4T1 and to investigate the mechanism.Methods The mouse breast cancer cells 4T1 were randomly divided into three groups:4T1+vehicle group, 4T1+exosome group and 4T1+exosome+Y294002 (an inhibitor of PI3K/Akt signaling pathway) group.4T1+vehicle group was added with 400μL se-rum-free medium, 4T1+exosome group was added with 400 μL serum-free medium containing 400 μg/mL exosome and 4T1+exosome+Y294002 group was added with 400 μL serum-free medium containing 400 μg/mL exosome and 5 μg/mL Y294002.We used the MTT, cell scratch test and Western blotting to examine the effect of BMSCs-derived exosome on the proliferation, migration and invasion abilities as well as the PI3K/Akt signaling pathway of 4T1 cells.Results The in-hibition rates of cell proliferation of 4T1+vehicle group, 4T1+exosome group and 4T1+exosome+Y294002 group were 0.713%±0.05%, 0.401%±0.03%and 0.459%±0.80%, respectively.Significant difference was found between 4T1+exosome group and 4T1+vehicle group, 4T1+exosome+Y294002 group (all P<0.05).The cell migration distance of 4T1+vehicle group, 4T1+exosome group and 4T1+exosome+Y294002 group was (388.0 ±36.1), (295.0 ± 34.2) and (275.0 ±63.5) μm, respectively.Significant difference was found between 4T1+exosome group and 4T1+vehicle group, 4T1+exosome+Y294002 group (all P<0.05).The OD values of p-AKT and β-catenin of 4T1+exo-some group were 0.3 ±0.11 and 0.3 ±0.08, they were 1.1 ±0.41 and 0.7 ±0.08 in 4T1+vehicle group, 0.4 ±0.13 and 0.3 ±0.07 in the 4T1+exosome+Y294002 group.Significant difference was found between 4T1+exosome group and 4T1+vehicle group, 4T1+exosome+Y294002 group (all P<0.05).Conclusion BMSCs-derived exosome can promote the proliferation, migration and invasion abilities of mouse breast cancer cells 4T1, which may be related to the up-regulation of PI3K/Akt signaling pathway.关键词
骨髓间充质干细胞/外泌体/乳腺肿瘤/乳腺癌/PI3K/Akt信号通路Key words
bone marrow mesenchymal stem cells/exosomes/breast neoplasms/breast carcinoma/PI3K/Akt signa-ling pathway分类
医药卫生
