世界中医药Issue(8)：1144-1147,1151,5.DOI:10.3969/j.issn.1673-7202.2015.08.004
木犀草素改善高脂诱导的 ApoE-／-小鼠非酒精性脂肪肝及动脉粥样硬化作用研究
Effects of Luteolin on High Fat Diet-induced ApoE-/-mice With Non-alcoholic Steatohepatitis and Atherosclerosis
摘要
Abstract
Objective:This study was to explore the possible effects of luteolin on ApoE-/-mice with non-alcoholic steatohepatitis induced by high fat diet as well as the efficacy on atherosclerosis.Methods:The model was induced by high fat diet,and luteolin were administered by gastric perfusion at 70 and 1 40 mg/kg qd for 1 2 weeks.The serum level of superoxide dismutase(SOD), malondialdehyde(MDA),glutathion peroxidase(GSH-Px)were measured.The contents of alanine transaminase(ALT),aspartate aminotransferase(AST)were measured in liver tissue of grout.The pathological changes of liver tissue were examined by HE stai-ning and the lipid accumulation in the liver and aortic root was tested by oil red O staining.The apoptotic hepatocytes were detec-ted by TUNEL assay and the expression levels of Bcl-2 and caspase-3 were observed by immunohistochemisty.Results:The results showed that Luteolin(70,1 40 mg/kg)improved the liver function significantly through reducing the level of ALT,AST,and in-creasing the content of SOD,GSH-Px and Bcl-2 while reducing the content of MDA,lipid accumulation,hepatic steatosis, TUNEL-positive cells and the expression of caspase-3.Conclusion:The results suggest that Luteolin may inhibit the liver injury and atherosclerosis induced by high fat diet by decreasing the oxidative stress.关键词
木犀草素/非酒精性脂肪肝/动脉粥样硬化/氧化损伤/ApoE-/-小鼠Key words
Luteolin/Non-alcoholic steatohepatitis/Atherosclerosis/Oxidative injury/ApoE-/-mice分类
医药卫生引用本文复制引用
罗云,卢珊,周平,孙桂波,孙晓波..木犀草素改善高脂诱导的 ApoE-／-小鼠非酒精性脂肪肝及动脉粥样硬化作用研究[J].世界中医药,2015,(8):1144-1147,1151,5.基金项目
国家自然科学基金项目（编号81374010）；国家科技重大专项课题，重大新药创制基金 （）
