世界中医药Issue(8)：1157-1162,6.DOI:10.3969/j.issn.1673-7202.2015.08.007
姜黄挥发油脂质体制备及其抗黄曲霉菌评价研究
Preparation of Turmeric Essential Oil Liposome and Research on Its Anti-Aspergillus flavus Efficacy
摘要
Abstract
Objective:To develop the nano-liposomal system of turmeric essential oil for inhibiting Aspergillus flavus growth and aflatoxin generation.Methods:The stabilized and bio-safe liposomes loading turmeric essential oil were prepared by the ethanol injection-ultrasonic emulsification method with lecithin and cholesterol as excipients.Based on the combination of single factor screening and orthogonal design,liposomes were obtained by means of the optimized technology.Subsequently,following with the physicochemical characterization,the anti-Aspergillus flavus activity of liposomes were evaluated.Results:With the ratio of leci-thin and cholesterol(1 0∶1 ,weight ratio),ratio of feeding drug and excipient(1 :5,weight ratio),1 mL/min of the ethyl alcohol adding speed and along with ultrasonic emulsification for 5 min,liposomes were prepared,exhibiting high encapsulation efficiency, suitable size distribution,and good storage stability.Particularly,after incubation with 2 mL essential oil liposome per 25 mL cul-ture medium for 1 0d,almost 1 00% of Aspergillus flavus growth could be suppressed,without aflatoxin production.Conclusion:The preparation of turmeric essential oil based on nano-system not only increases the stability and homogeneity of the system but guarantees the efficacy of anti-Aspergillus flavus.关键词
姜黄挥发油/纳米/脂质体/黄曲霉菌/黄曲霉毒素Key words
Turmeric essential oil/Nanoparticles/Liposome/Aspergillus flavus/Aflatoxin分类
医药卫生引用本文复制引用
胡一晨,孔维军,杨美华..姜黄挥发油脂质体制备及其抗黄曲霉菌评价研究[J].世界中医药,2015,(8):1157-1162,6.基金项目
十二五国家科技重大专项重大新药创制“中药质量安全检测和风险控制技术平台” （）
