世界中医药Issue(8)：1173-1176,4.DOI:10.3969/j.issn.1673-7202.2015.08.010
黄精属药用植物 DNA 条形码鉴定研究
Identification Study of DNA Barcode Sequences in the Medicinal Plants of Polygonatum
摘要
Abstract
Objective:To evaluate the identification efficiencies of the DNA barcode sequences for the medicinal plants of Polygo-natum.Methods:The chloroplast psbA-trnH,matK,and rbcL and nuclear ITS and ITS2 of 44 Polygonatum samples were ampli-fied using the universal primers.The intra-and inter-specific genetic distance and barcoding gap were analyzed.The identification efficiencies of the sequences were calculated by BLAST and Nearest Distance methods.Results:ITS and ITS2 of Polygonatum were difficult to obtain using the universal primers.The available rbcL were the highest while the psbA-trnH was the lowest.The intra-and inter-specific genetic distances were all low and no obvious barcode gap existed in three candidate loci.The identification efficiencies of the three chloroplast regions were all low according to BLAST and Nearest Distance methods.Conclusion:The DNA barcode sequences did not meet the demands of identifying the medicinal plants of Polygonatum,and the complete chloroplast ge-nome sequences(CP)or the suitable regions screened from CP may be applied to identify the medicinal plants of Polygonatum in the future.关键词
黄精属/分子鉴定/DNA 条形码Key words
Polygonatum/Molecular identification/DNA barcode分类
医药卫生引用本文复制引用
杨培,周红,辛天怡,马双姣,段宝忠,姚辉..黄精属药用植物 DNA 条形码鉴定研究[J].世界中医药,2015,(8):1173-1176,4.基金项目
国家科技支撑计划（编号2011 BAI07B08）；国家自然科学基金项目 （）
