世界中医药Issue(8)：1222-1226,5.DOI:10.3969/j.issn.1673-7202.2015.08.023
通心络对2型糖尿病大鼠肾脏血管紧张素Ⅱ表达的影响
Effects of Tongxinluo on the Renal Angiotensin Ⅱ Expression in Type 2 Diabetic Rats
摘要
Abstract
Objective:To observe the renal angiotensin Ⅱ expression in type 2 diabetic rats,to investigate the effect and the possi-ble mechanism of Tongxinluo on glomerular hyperfiltration in early diabetes.Methods:The rat model of type 2 diabetes mellitus was induced by injection of low-dose of streptozocin after having high-fat diet for 4 weeks,and they were randomly divided into 4 groups—normal group,diabetic group,valsartan treated group (1 0 mg·kg -1 ·d -1 )and Tongxinluo treated group(0.4 mg· kg -1 ·d -1 );the level of fasting blood glucose(FBG),urinary albumin excretion rate(UAER)and the renal function included se-rum creatinine(Scr),blood urea nitrogen(BUN)and creatinine clearance rate (Ccr)were measured every 4 weeks;After 8 weeks,kidney weight/body weight ratio(KW/BW)was calculated and the renal expression of angiotensin Ⅱ and angiotensin Ⅱtype 1 receptor(AT1 R)was determined by western blot and real-time PCR.Results:The level of UAER,Scr,BUN,Ccr,KW/BW,AngⅡand AT1 R expression in Tongxinluo treated group was lower than it in diabetic group(P <0.05)while FBG had no significant alteration(P >0.05).Conclusion:Tongxinluo can improve glomerular hyperfiltration,urinary albumin excretion,kid-ney hypertrophia and the renal function in type 2 diabetic rats.The mechanism could be related to inhibition of AngⅡand AT1 R expression.关键词
通心络/2 型糖尿病/肾小球高滤过/血管紧张素ⅡKey words
Tongxinluo/Type 2 diabetes mellitus/Glomerular hyperfiltration/Angiotensin Ⅱ分类
医药卫生引用本文复制引用
李娇阳,高彦彬,周盛楠,邹大威,朱智耀,张娜,王馨瑶,崔方强,刘静..通心络对2型糖尿病大鼠肾脏血管紧张素Ⅱ表达的影响[J].世界中医药,2015,(8):1222-1226,5.基金项目
国家重点基础研究发展计划课题（“973”计划） （）
