色谱Issue(9)：951-956,6.DOI:10.3724/SP.J.1123.2015.05032
万古霉素及其杂质的亲水作用色谱分析
Analysis of vancomycin and its related impurities by hydrophilic interaction liquid chromatography
摘要
Abstract
The current reversed⁃phase liquid chromatography as the main analytical method for vancomycin is inappropriate due to its poor polarity selectivity and mass spectrometry compati⁃bility. Hydrophilic interaction chromatography ( HILIC) method has been verified to provide the glycopeptide substances with good retention and polarity selectivity, therefore, an HILIC meth⁃od was developed for the analysis of vancomycin and its related impurities including norvanco⁃mycin, desvancosaminyl vancomycin, dedichloro vancomycin and crystalline degradation prod⁃uct ( CDP⁃1) . In this work, a systematic evaluation of retention behavior of vancomycin and its related impurities in hydrophilic interaction chromatography was performed and the influences of stationary phase, acetonitrile content, buffer salt and pH of mobile phase were studied. According to the results, Click XIon column was selected as stationary phase and ammonium formate was additive in the mobile phase. Vancomycin and its related impurities were totally separated under the optimization conditions. It is concluded that hydrophilic interaction chroma⁃tography may be a new strategy for the separation of vancomycin and its structural analogues.关键词
亲水作用色谱/万古霉素/糖肽类抗生素/杂质分析Key words
hydrophilic interaction chromatography ( HILIC )/vancomycin/glycopeptides antibiotics/impurities analysis分类
化学化工引用本文复制引用
闫竞宇,郭志谋,丁俊杰,沈爱金,王纪霞,金高娃,梁鑫淼..万古霉素及其杂质的亲水作用色谱分析[J].色谱,2015,(9):951-956,6.基金项目
国家自然科学基金项目（21207130）． （）
