色谱Issue(9)：974-980,7.DOI:10.3724/SP.J.1123.2015.04028
超高效液相色谱-二极管阵列检测-串联质谱法测定茶叶中15种黄酮醇糖苷类化合物
Determination of flavonol glycosides in tea samples by ultra-high performance liquid chromatography-photodiode array detection-tandem mass spectrometry
摘要
Abstract
An ultra⁃high performance liquid chromatography⁃photodiode array detection⁃tandem mass spectrometry ( UPLC⁃PDA⁃MS/MS) method was developed for the determination of flavo⁃nol glycosides in tea samples. The chromatographic separation was performed on an UPLC HSS T3 column by gradient elution with the mobile phases of acetonitrile and water both containing 0�1%( v/v) formic acid. A total of 15 flavonol glycosides which include 3 myricetin glycosides, 6 quercetin glycosides and 6 kaempferol glycosides were positively identified in green and black tea samples by comparing the retention times and mass spectra of the samples with standards and publications. The quantities of flavonol glycosides were relatively calculated with the stand⁃ard quercetin⁃3⁃rhamnosylglucoside ( Q⁃GRh) which was calibrated with external quantification method using multi⁃reaction monitoring ( MRM ) mode. The results showed that there were different flavonol glycoside distributions in green tea and black tea. The total amount of flavonol glycosides in green tea was 1�7 times of that in black tea. The major flavonol glycosides in green tea were myricetin⁃3⁃galactoside ( M⁃Ga) , myricetin⁃3⁃glucoside ( M⁃G) , quercetin⁃3⁃glucosyl⁃rhamnosyl⁃galactoside ( Q⁃GaRhG ) , quercetin⁃3⁃glucosyl⁃rhamnosyl⁃glucoside ( Q⁃GRhG) , kaempferol⁃3⁃glucosyl⁃rhamnosyl⁃galactoside ( K⁃GaRhG ) and kaempferol⁃3⁃glucosyl⁃rhamnosyl⁃glucoside ( K⁃GRhG) , but for black tea, the major flavonol glycosides were querce⁃tin⁃3⁃rhamnosylglucoside ( Q⁃GRh) , quercetin⁃3⁃glucoside ( Q⁃G) , kaempferol⁃3⁃rhamnosylglu⁃coside (K⁃GRh) and kaempferol⁃3⁃galactoside (K⁃Ga). The present method is accurate, con⁃venient for the rapid identification of flavonol glycosides and analysis of constituent distribution for green and black teas.关键词
超高效液相色谱-二极管阵列检测-串联质谱/黄酮醇糖苷类化合物/茶叶Key words
ultra-high performance liquid chromatography-photodiode array detection-tandem mass spectrometry ( UPLC-PDA-MS/MS)/flavonol glycosides/teas分类
化学化工引用本文复制引用
王智聪,沙跃兵,余笑波,梁月荣..超高效液相色谱-二极管阵列检测-串联质谱法测定茶叶中15种黄酮醇糖苷类化合物[J].色谱,2015,(9):974-980,7.基金项目
浙江省公益技术应用研究项目（2015C37070）． （）
