生物技术通报Issue(9)：91-96,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.09.012
Cry1Ab/Cry1Ah杂合蛋白构建与功能研究
Creation of Cry1Ab/Cry1Ah Hybrid Proteins and Its Functional Analysis
摘要
Abstract
Cry1A is the most widely used insecticidal protein, and the domain swapping in the reported Cry1A family frequently occurs. In this paper, we investigated the Lepidoptera pests’highly active protein Cry1Ab and Cry1Ah, and constructed the hybrid protein AhAhAb from Cry1Ab/Cry1Ah and bioassayed the activities. The results showed that the domain swapping of two proteins Cry1Ab and Cry1Ah resulted in the insecticidal activities significantly changed. Compared with the origin proteins, hybrid protein AhAhAb lost the insecticidal activity to Heliothis armigera, caused the insecticidal activity to Ostrinia nubilalis, Plutella xylostella reduced. We then modeled Cry1Ah domain I, and compared the structure and the surface properties with other Cry1A proteins by bioinformatics methods. The results indicated that domain I of Cry1Ah and Cry1Ab had identical carbon skeleton and secondary structure but varied surface and potential distribution. The further studies on the reasons of the activity’s difference between hybrid protein AhAhAb and Cry1Ab or Cry1Ah will give enlightenment for unclosing the evolution pattern of insecticidal specificity of Cry1A family proteins.关键词
Cry1Ah/结构域I/杀虫活性Key words
Cry1Ah/domain I/insecticidal activity引用本文复制引用
徐曼,蒋健,束长龙,张杰,宋福平..Cry1Ab/Cry1Ah杂合蛋白构建与功能研究[J].生物技术通报,2015,(9):91-96,6.基金项目
国家转基因专项 （）
