生物技术通报Issue(9)：105-110,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.09.014
甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究
Correlations Between Sucrose Accumulation and Activities of SPS,SS at Processing Maturing Stage of Sugarcane
摘要
Abstract
The correlations between sucrose accumulation and activities of 3 enzymes viz, sucrose phosphate synthase(SPS), sucrose synthase in the synthesis direction(SS-s), and sucrose synthase in the cleavage direction(SS-c)were studied using the leaf+1 and 7 internodes of early-maturing cultivar GT28 and early-intermediate-maturing cultivar ROC22 at processing maturing stage of sugarcane. The results indicated that the activities of SPS, SS-s and SS-c in the leaf+1 were higher than those in stalks of both GT28 and ROC22, suggesting that the leaf+1 was vigorous and active for physiological metabolism, which was the substantial basis for continuous sucrose accumulation in stalks. The SPS activity in internode was significantly and positively correlated with the brix, total soluble sugar and sucrose content of internode, and it was higher in GT28 than in ROC22. Meanwhile, SS-s activity was significantly and negatively correlated with the brix and sucrose content in internode, and it was lower in GT28 than in ROC22. However, SS-c was significantly and negatively correlated with brix, total soluble sugar and sucrose content in the internode of ROC22, while positively in GT28 although it was not significant statistically. This suggests that SPS and SS-s activities promote the sucrose accumulation in the immature internodes;with the internode maturing, sucrose content might be an important regulatory factor to convert the SS activity from synthesis to cleavage direction.关键词
甘蔗/蔗糖磷酸合成酶/蔗糖合成酶/蔗糖含量/相关性分析Key words
sugarcane/sucrose phosphate synthase/sucrose synthase/sucrose accumulation/correlation analysis引用本文复制引用
牛俊奇,黄静丽,赵文慧,杨丽涛,李杨瑞..甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究[J].生物技术通报,2015,(9):105-110,6.基金项目
国家“863”计划课题（2013AA102604），广西自然科学基金项目（2011GXNSFF018002，2013NXNSFAA019073），国家国际合作项目（2009DFA30820，2013DFA31600），广西八桂学者和特聘专家专项经费资助 （）
