生物技术通报Issue(9)：177-182,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.09.025
高产铁载体假单胞菌的筛选及其对铁氧化物的利用
Screening of Pseudomonas Strains Producing High-yield Siderophore and Its Utilization of Iron Hydroxides
摘要
Abstract
The aim of this study is to isolate the microorganisms producing high-yield siderophore, investigate antimicrobial activity of siderophore against pathogenic bacteria, and study the effect of siderophore on the utilization of insoluble poorly crystalline iron hydroxides (PICH). CAS method was used for isolating microorganisms producing high-yield siderophore. Agar diffusion method and growth inhibition assay were used to test antimicrobial activities of siderophore. Sequence alignment of 16S rRNA gene was used for strain identification. Bacterial growth was measured to investigate the utilization of insoluble PCIH by siderophore. Totally, 172 siderophore-producing strains were isolated from soil samples, including 13 potentially strong siderophore producers(PSSP). The analysis of antimicrobial activities of siderophore showed that only the supernatant of Z158 culture had a pronounced inhibiting effect on the growth of Staphylococcus aureus, Micrococcus luteus, Proteus vulgaris, and Vibrio parahemolyticus, and the inhibition efficiency was 51.3%, 50.2%, 37.1%, and 28.0%, respectively. Homology analysis of 16S rRNA gene sequence identified strain Z158 as Pseudomonas aeruginosa. Moreover, with insoluble PICH as sole iron source, the biomass of Z158 increased by 46.1%at 24 h after incubation. In conclusion, the siderophore secreted by P. aeruginosa Z158 could inhibit the growth of pathogenic bacteria and acquire iron element from insoluble PCIH.关键词
铁载体/铜绿假单胞菌/抑菌/未定型铁氧化物Key words
siderophore/Pseudomonas aeruginosa/antimicrobial activity/poorly crystalline iron hydroxides引用本文复制引用
朱慧明,张彦,杨洪江..高产铁载体假单胞菌的筛选及其对铁氧化物的利用[J].生物技术通报,2015,(9):177-182,6.基金项目
由天津市科技支撑计划重点项目 （）
