生物技术通报Issue(9)：209-217,9.DOI:10.13560/j.cnki.biotech.bull.1985.2015.09.030
大肠杆菌K235能量代谢扰动影响聚唾液酸合成的研究
Influence of Perturbation of Energy Metabolism in Escherichia coli K235 on Biosynthesis of Polysialic Acid
摘要
Abstract
Different medium volumes, carbon sources with varied redox states and adding nicotinic acid, and the precursor of NAD+were used to adjust the intracellular energy charge and reduction of Escherichia coli in order to investigate the relationships between biosynthesis of polysialic acid and intracellular nucleotides. The results showed that the higher the production of polysialic acid was, the lower UTP was, but higher UMP was. Meanwhile, energy charge level was lower because the synthesis of UTP and CTP consumed a lot of ATP. Using sorbitol as carbon source, more polysialic acid was produced than those of fermentation with glucose, xylose and sodium gluconate due to the increase of NADH. Addition of nicotinic acid to the fermentation medium elevated intracellular NAD+, which led to the raise of intracellular oxidative stress, sequentially the decrease of the reducing metabolites such as polysialic acid, succinate and lactate, and even the vanish of them.关键词
大肠杆菌/聚唾液酸/能荷/核苷酸/氧化还原势Key words
Escherichia coli/polysialic acid/energy charge/nucleotides/redox potential引用本文复制引用
闫霞,吴剑荣,郑志永,詹晓北..大肠杆菌K235能量代谢扰动影响聚唾液酸合成的研究[J].生物技术通报,2015,(9):209-217,9.基金项目
国家“863”计划项目（2012AA021505），江苏省自然科学基金项目 （）
