实用医学杂志2015，Vol.31Issue(14)：2271-2275,5.DOI:10.3969/j.issn.1006-5725.2015.14.010
基因在食管鳞癌组织和血浆中的甲基化特征及临床意义
The characteristics of EPPB41L3 methylation in esophageal squamous cell carcinoma tissues and in plasma and the clinical significance of this methylation
摘要
Abstract
Objective To detect EPPB41L3 methylation frequency difference between esophageal squamous cell carcinoma (ESCC) tissues and the normal tissues and between ESCC patients′plasma and healthy volenteers′plasma, and to analyze the correlation with clinicopathological parameters. Methods We collected esophageal squamous cell carcinoma tissues (n = 42 patients) and adjacent surrounding normal tissues (n = 42 patients), and plasma from 42 patients with ESCC and from 50 healthy individuals. We used methylation specific PCR (MSP) combined with agarose gel electrophoresis to detect the methylation status of the EPB41L3. We used the SPSS 13.0 software for statistical analysis by χ2 test and Fisher′s exact test. Results EPB41L3 frequency of methylation was significantly higher in tumor tissues than in the adjacent tissues (59.5% vs. 4.8%), the difference was statistically significant (χ2 = 28.873, P < 0.001). For plasma, EPB41L3 methylation frequency was 31.0%in cancer patients, while was not detectable in the healthy volunteers. Methylation of EPB41L3 in tissues was more frequently found in patients with tumor size of ≥ 5 cm or T3 than in patients with tumor size of < 5 cm or T1-2. Conclusions The methylation frequency of EPB41L3 is higher in ESCC tissues than in control normal tissues, and higer in plasma from ESCC patients than that from the healthy volunteers. EPB41L3 methylation is more frequently found in patients with more advanced disease.关键词
食管肿瘤/甲基化/EPPB41L3基因/表观遗传学Key words
Esophageal squamous cell carcinoma/EPB41L3 gene/DNA methylation/Epigenetics引用本文复制引用
熊威斌,李许锋,蒋春雨,张积仁,周福有,余力,郑燕芳..基因在食管鳞癌组织和血浆中的甲基化特征及临床意义[J].实用医学杂志,2015,31(14):2271-2275,5.基金项目
国家自然科学基金青年项目(编号:81202073) （编号:81202073）
