中国组织工程研究Issue(33)：5279-5283,5.DOI:10.3969/j.issn.2095-4344.2015.33.007
腰椎髓核组织中缺氧诱导因子1α的表达：与凋亡率正相关
Expression of hypoxia-inducible factor 1 alpha and its relationship to apoptosis in human lumbar nucleus pulposus
刘兴振 1金文杰 1沈康平 1傅智轶 1吴玉杰1
作者信息
- 1. 上海交通大学医学院附属第三人民医院骨科，上海交通大学医学院创伤医学研究所，上海市 201900
- 折叠
摘要
Abstract
BACKGROUND:Under hypoxic environment, hypoxia-inducible factor 1α plays a dualregulatory role in cel apoptosis. Severity of hypoxia is the key to determine whether cels appear to have apoptosis or adapt to survive. When the cels are exposed to chronic or extreme hypoxia, a lack of protection mechanisms from hypoxia-inducible factor-1α can induce cel apoptosis. OBJECTIVE: To research the expression of hypoxia-inducible factor 1α in human lumbar nucleus pulposus of different herniated types and its relationships with cel apoptosis. METHODS: The nucleus pulposus was harvested from 60 cases of herniation of lumbar intervertebral discs, L4-5 in 41 cases and L5-S1 in 19 cases. The nucleus pulposus tissues were equaly divided into protruded and sequestered groups. Meanwhile, the nucleus pulposus tissues from another 10 cases of lumbar spine fracture were taken as control group. Expression of hypoxia-inducible factor 1α and apoptosis of lumbar nucleus pulposus cels were observed and detected with immunohistochemical technique and TUNEL method. Correlation of hypoxia-inducible factor 1α and apoptosis in human lumbar nucleus pulposus of different herniated types was analyzed. RESULTS AND CONCLUSION: The expression of hypoxia-inducible factor 1α was visualized in each case, but it was significantly higher in the sequestered group than in the protruded group and control group (P < 0.01). Apoptosis of nucleus pulposus cels were found in al the three groups, but the apoptotic rate was also higher in the sequestered group than in the protruded group and control group (P < 0.01). Expression of hypoxia-inducible factor 1α was positively correlated with cel apoptosis in human lumbar nucleus pulposus (P < 0.01). Overal, the expression of hypoxia-inducible factor1α in degenerative human lumbar nucleus pulposus is associated with herniated types, which is the highest in the sequestered type. The relationship between hypoxia-inducible factor 1α and apoptosis is positive.关键词
组织构建/组织工程/髓核/腰椎/缺氧诱导因子1α/凋亡Key words
Lumbar Vertebrae/Hypoxia-Inducible Factor I/alpha Subunit/Apoptosis分类
医药卫生引用本文复制引用
刘兴振,金文杰,沈康平,傅智轶,吴玉杰..腰椎髓核组织中缺氧诱导因子1α的表达：与凋亡率正相关[J].中国组织工程研究,2015,(33):5279-5283,5.
